氮化碳负载高负载量铜单原子纳米酶的类过氧化物酶活性及其用于没食子酸的比色检测。

High-loading Cu single-atom nanozymes supported by carbon nitride with peroxidase-like activity for the colorimetric detection of tannic acid.

机构信息

College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637000, China.

出版信息

Talanta. 2023 May 15;257:124387. doi: 10.1016/j.talanta.2023.124387. Epub 2023 Feb 21.

DOI:10.1016/j.talanta.2023.124387

PMID:36841014

Abstract

The design of nanozymes with high metal atom loading is of great significance to improve enzyme activity and is also the key to furthering the construction of highly sensitive colorimetric sensors. In this work, a colorimetric sensor for the quantitative analysis of tannic acid (TA) was developed based on two-dimensional carbon nanosheet carbon nitride (CN)-supported Cu single-atom nanozymes (Cu/CN). Cu/CN was synthesized by supramolecular preorganization and calcination, with an ultrathin nanosheet structure and a high density of Cu active sites, with a Cu loading of up to 14.3 wt%. Benefiting from the above characteristics, Cu/CN exhibits peroxidase-mimicking activity and excellent catalytic performance. Therefore, a colorimetric sensor was constructed for the fast and sensitive quantitative analysis of TA with good linearity in the range of 0.09-3.2 μM and a low detection limit of 30 nM. Furthermore, the sensor was successfully applied to the analysis of TA in tea samples of different varieties. This work sheds new light on the design of nanozymes with a high density of active sites and the analysis of TA in real environments.

摘要

高金属原子负载量的纳米酶的设计对于提高酶活性具有重要意义，也是进一步构建高灵敏度比色传感器的关键。在这项工作中，基于二维碳纳米片氮化碳（CN）负载的铜单原子纳米酶（Cu/CN），开发了一种用于单宁酸（TA）定量分析的比色传感器。Cu/CN 通过超分子预组织和煅烧合成，具有超薄纳米片结构和高密度的 Cu 活性位点，Cu 负载量高达 14.3wt%。得益于上述特点，Cu/CN 表现出过氧化物酶模拟活性和优异的催化性能。因此，构建了一种比色传感器，用于 TA 的快速灵敏定量分析，在 0.09-3.2 μM 范围内具有良好的线性关系，检测限低至 30 nM。此外，该传感器成功应用于不同品种茶叶样品中 TA 的分析。这项工作为高活性位点密度的纳米酶设计和实际环境中 TA 的分析提供了新的思路。