基于靶标响应水凝胶和HO蚀刻金纳米棒的透明质酸酶多色生物传感器。

Multicolor biosensor for hyaluronidase based on target-responsive hydrogel and etching of gold nanorods by HO.

作者信息

Wang Weiquan, Xu Yunpeng, Hou Jie, Li Zhixin, Luo Fang, He De, Lin Zhenyu

机构信息

Department of General Surgery, The Second Affiliated Hospital of Shenzhen University, Guangdong, 518000, PR China.

Department of Nephrology, The Second Affiliated Hospital of Shenzhen University, Guangdong, 518000, PR China.

出版信息

Talanta. 2023 May 15;257:124367. doi: 10.1016/j.talanta.2023.124367. Epub 2023 Feb 16.

DOI:10.1016/j.talanta.2023.124367

PMID:36841016

Abstract

Hyaluronidase (HAase) is a potential tumor biomarker for diseases of the digestive tract and nervous system, the development of simple and sensitive techniques for HAase determination is urgent needed. Gold nanorods (Au NRs) can be etched by HO with high efficiency and display color changing. In this work, a HAase-responsive hydrogel system had been designed and the amount of HO spilled from the system had a close relationship with the amount of HAase, then the spilled HO had been applied to etch Au NRs. The color change of the solution was used to realize semi-quantitative determination of HAase. Furthermore, the longitudinal peak shift of Au NRs had a linear correlation with the concentration of HAase in the range of 10-60 U/mL (within 40 min) and the limit of detection (LOD) was 3.8 U/mL (S/N = 3), which can be used to realize accurate quantitative analysis of HAase. The proposed method has been applied to monitor HAase in serum of pancreatic cancer patients with satisfied results.

摘要

透明质酸酶（HAase）是消化系统和神经系统疾病的一种潜在肿瘤生物标志物，因此迫切需要开发简单且灵敏的HAase检测技术。金纳米棒（Au NRs）可被HO高效蚀刻并呈现颜色变化。在本研究中，设计了一种HAase响应水凝胶体系，从该体系中溢出的HO量与HAase量密切相关，然后将溢出的HO用于蚀刻Au NRs。利用溶液的颜色变化实现HAase的半定量检测。此外，在10 - 60 U/mL范围内（40分钟内），Au NRs的纵向峰位移与HAase浓度呈线性相关，检测限（LOD）为3.8 U/mL（S/N = 3），可用于实现HAase的准确定量分析。所提出的方法已应用于监测胰腺癌患者血清中的HAase，结果令人满意。