定制过氧化物模拟酶双功能纳米复合材料：在真实食品样品和活细胞中胆固醇和过氧化氢的双模式电光谱筛选。

Tailoring of peroxidase mimetics bifunctional nanocomposite: Dual mode electro-spectroscopic screening of cholesterol and hydrogen peroxide in real food samples and live cells.

机构信息

Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 106, Taiwan, ROC.

School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.

出版信息

Food Chem. 2023 Jul 15;414:135747. doi: 10.1016/j.foodchem.2023.135747. Epub 2023 Feb 20.

DOI:10.1016/j.foodchem.2023.135747

PMID:36841102

Abstract

A simple and rapid screening of biomarkers in clinical and food matrices is urgently needed to diagnose cardiovascular diseases. The cholesterol (Chol) and hydrogen peroxide (HO) are critical bio-indicators, which require more inventive detection techniques to be applied to real food, and bio-samples. In this study, a robust dual sensor was developed for Chol and HO using hybrid catalyst. Bovine serum albumin (BSA)-capped nanocatalyst was potentially catalyzed 3,3',5,5'-tetramethylbenzidine (TMB), and HO. The enzymatic nanoelectrocatalyst delivered a wide range of signaling concentrations from 250 nM to 3.0 mM and 100 nM to 10 mM, limit of detection (LOD) of 53.2 nM and 18.4 nM for Chol and HO. The cholesterol oxidase-BSA-AuNPs-metal-free organic framework (ChOx-BSA-AuNPs-MFOF) based electrode surface effectively operated in live-cells and real-food samples. The enzymatic sensor exhibits adequate recovery of real-food samples (96.96-99.44%). Finally, the proposed system is a suitable choice for the potential applications of Chol and HO in clinical and food chemistry.

摘要

为了诊断心血管疾病，迫切需要在临床和食品基质中对生物标志物进行简单快速的筛选。胆固醇（Chol）和过氧化氢（HO）是关键的生物指标，需要更多创新的检测技术应用于实际食品和生物样本。在本研究中，使用混合催化剂开发了用于 Chol 和 HO 的强大双传感器。牛血清白蛋白（BSA）封端的纳米催化剂可潜在地催化 3,3',5,5'-四甲基联苯胺（TMB）和 HO。该酶纳米电催化剂提供了从 250 nM 到 3.0 mM 和从 100 nM 到 10 mM 的宽信号浓度范围，Chol 和 HO 的检测限（LOD）分别为 53.2 nM 和 18.4 nM。基于胆固醇氧化酶-BSA-金纳米颗粒-无金属有机框架（ChOx-BSA-AuNPs-MFOF）的电极表面可在活细胞和实际食品样品中有效运行。该酶传感器对实际食品样品具有足够的回收率（96.96-99.44%）。最后，该系统是 Chol 和 HO 在临床和食品化学中潜在应用的合适选择。