State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Quality and Health of Tianjin, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.
Mikrochim Acta. 2023 Nov 23;190(12):479. doi: 10.1007/s00604-023-06073-7.
A novel peroxidase-like nanozyme has been constructed by decorating two-dimensional TiCT nanosheets (TiCT NSs) with gold nanoparticles (AuNPs) to develop a colorimetric and photothermal dual-mode immunosensor. The TiCT/AuNPs nanocomposite-catalyzed 3,3',5,5'-tetramethylbenzidine (TMB)-HO reaction system produces the one-electron oxidation product of TMB (oxTMB), which exhibits color change and strong near-infrared (NIR) laser-driven photothermal effect at 808 nm laser irradiation. Given these characteristics, the developed immunosensor achieves ultrasensitive dual-mode detection of zearalenone (ZEN) by measuring colorimetric and photothermal signals with a microplate reader and a portable infrared thermometer, respectively. Under optimal working conditions, the limit of detection (LOD) of ZEN is 0.15 pg mL for the colorimetric mode and 0.48 pg mL for the photothermal mode. In the analysis of actual contaminated cereals samples, the test result of this method was consistent with that of UPLC-MS/MS. The proposed colorimetric and photothermal dual-mode immunosensor offers a new strategy for the low-cost detection of hazardous substances. The application of a widely used household infrared thermometer makes the signal readout more convenient, which provides great prospects in food safety and environment inspection applications.
一种新型的过氧化物酶样纳米酶通过在二维 TiCT 纳米片（TiCT NSs）上修饰金纳米颗粒（AuNPs）构建，用于开发比色和光热双模式免疫传感器。TiCT/AuNPs 纳米复合材料催化 3,3',5,5'-四甲基联苯胺（TMB）-HO 反应体系产生 TMB 的单电子氧化产物（oxTMB），在 808nm 激光照射下表现出色变和强近红外（NIR）激光驱动的光热效应。鉴于这些特性，所开发的免疫传感器通过分别使用微孔板读数器和便携式红外温度计测量比色和光热信号，实现了玉米赤霉烯酮（ZEN）的超灵敏双模式检测。在最佳工作条件下，ZEN 的比色模式的检测限（LOD）为 0.15pg mL，光热模式的 LOD 为 0.48pg mL。在实际污染谷物样品的分析中，该方法的测试结果与 UPLC-MS/MS 的结果一致。所提出的比色和光热双模式免疫传感器为危险物质的低成本检测提供了一种新策略。广泛使用的家用红外温度计的应用使信号读取更加方便，这在食品安全和环境检测应用中具有广阔的前景。
