Jing Huiyuan, Song Yuzhen, Li Huawei, Duan Erzhen, Liu Jie, Ke Wenting, Tao Ran, Li Yang, Zhao Pandeng, Wang Jinhe, Cao Sufang, Wang Haihua, Sun Yanting, Zhang Yan
Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
Virology. 2023 Apr;581:15-25. doi: 10.1016/j.virol.2023.02.006. Epub 2023 Feb 22.
HnRNP K is a well-known member of HnRNP family proteins that has been implicated in the regulation of protein expression. Currently, the impact of HnRNP K on the reproduction cycle of a broad range of virus were reported, while the precise function for PRRSV was lacking. In this study, we determined that both PRRSV infection and ectopic expression of N protein induced an enrichment of HnRNP K in the cytoplasm. Using RNA pulldown and RNA immunoprecipitation, we described the interactions between the KH2 domain of HnRNP K and cytosine-rich sequences (CRS) in PRRSV genomic RNA corresponding to Nsp7α coding region. Meanwhile, overexpression of HnRNP K inhibited viral gene expression and PRRSV replication, while silencing of HnRNP K resulted in an increased in virus yield. Taken together, this study assists in the understanding of PRRSV-host interactions, and the development of vaccines based on viral genome engineering.
异质性核糖核蛋白K（HnRNP K）是HnRNP家族蛋白中一个著名的成员，它与蛋白质表达的调控有关。目前，已有报道称HnRNP K对多种病毒的复制周期有影响，但对猪繁殖与呼吸综合征病毒（PRRSV）的具体作用尚不清楚。在本研究中，我们发现PRRSV感染和N蛋白的异位表达均能诱导HnRNP K在细胞质中富集。通过RNA下拉和RNA免疫沉淀，我们描述了HnRNP K的KH2结构域与PRRSV基因组RNA中对应于Nsp7α编码区的富含胞嘧啶序列（CRS）之间的相互作用。同时，HnRNP K的过表达抑制了病毒基因表达和PRRSV复制，而HnRNP K的沉默则导致病毒产量增加。综上所述，本研究有助于理解PRRSV与宿主的相互作用，以及基于病毒基因组工程的疫苗开发。
