Popov Hristo, Stoyanov George S, Ghenev Peter
General and Clinical Pathology, Forensic Medicine and Deontology, Medical University of Varna, Varna, BGR.
General and Clinical Pathology, St. Marina University Hospital, Varna, BGR.
Cureus. 2023 Jan 24;15(1):e34147. doi: 10.7759/cureus.34147. eCollection 2023 Jan.
Introduction Urothelial carcinomas represent a distinct group of malignancies with a high recurrence potential. Multiple studies have established a set of interactions between the tumor cells of urothelial neoplasms and the extracellular matrix regarding invasion and tumor progression. In the present study, we evaluated the expression of fibroblast growth factor-2 (FGF2) in early-stage urothelial carcinomas of the urinary bladder (pTa and pT1) regarding the invasive potential of these tumors. Materials and methods A retrospective non-clinical approach was utilized for the study. Tumor tissue sections used for the initial diagnosis were stained by immunohistochemical means with an anti-FGF2 antibody and the expression within the extracellular matrix was evaluated using a histo-score (h-score). Statistical parameters regarding tumor invasion, FGF2 expression pattern and levels, patient demographic characteristics, and disease recurrence were analyzed for significance. Results A total of 163 cases were analyzed, with an h-score of 110 determined as the optimal cut-off value for invasive potential regarding FGF2 expression, with a sensitivity of 75.4% and a specificity of 78.9%. No statistical correlation was established between the demographic profile of the patients and the occurrence of disease recurrence. Conclusion Based on our results, the study of tumor-extracellular matrix interactions in regards to FGF2 expression is a promising field, at least in urothelial malignancies of the urinary bladder, in regards to tumor invasive potential, while it remains unestablished how these interactions affect metastatic potential.
引言 尿路上皮癌是一组具有高复发潜能的独特恶性肿瘤。多项研究已经证实尿路上皮肿瘤的肿瘤细胞与细胞外基质之间在侵袭和肿瘤进展方面存在一系列相互作用。在本研究中，我们评估了成纤维细胞生长因子2（FGF2）在膀胱早期尿路上皮癌（pTa和pT1）中的表达情况，以了解这些肿瘤的侵袭潜能。材料与方法 本研究采用回顾性非临床研究方法。用于初始诊断的肿瘤组织切片用抗FGF2抗体进行免疫组织化学染色，并使用组织评分（h评分）评估细胞外基质中的表达情况。分析了与肿瘤侵袭、FGF2表达模式和水平、患者人口统计学特征以及疾病复发相关的统计参数的显著性。结果 共分析了163例病例，确定h评分为110作为FGF2表达侵袭潜能的最佳临界值，敏感性为75.4%，特异性为78.9%。患者的人口统计学特征与疾病复发的发生之间未建立统计学相关性。结论 根据我们的结果，关于FGF2表达的肿瘤-细胞外基质相互作用的研究是一个有前景的领域，至少在膀胱尿路上皮恶性肿瘤中，就肿瘤侵袭潜能而言，而这些相互作用如何影响转移潜能仍未明确。
