McDaniel Amanda, Freimark Bruce, Navarro Cebrina, Von Rothstein Kristin, Gonzalez Dacia, Linder Keith, Nuccitelli Richard
Department of Biology, Pulse Biosciences, Hayward, CA, United States.
Department of Dermatopathology, Linder Pathology Services, Raleigh, NC, United States.
Front Oncol. 2023 Feb 8;12:948472. doi: 10.3389/fonc.2022.948472. eCollection 2022.
Nano-Pulse Stimulation™ Therapy (NPS™) is a new, bioelectric modality that applies ultrashort pulses of electric energy to trigger regulated cell death in treated tissues. Instead of initiating necrosis by heating or freezing, NPS therapy permeabilizes intracellular organelles to activate the cell's own self-destruct pathway of programmed or regulated cell death. Unlike cryotherapies that can both damage structural tissues and diffuse into the periphery beyond the margins of the lesion, NPS only affects cells within the treated zone leaving surrounding tissue and acellular components unaffected.
We generated melanoma tumors in mice by injecting B16-F10 cells intradermally and compared the efficacy and resulting skin damage from Nano-Pulse Stimulation Therapy with that of cryoablation in clearing these tumors.
The results of the study demonstrate that NPS is superior at clearing B16-F10 melanoma lesions. NPS permanently eliminated up to 91% of all tumor lesions with a single treatment compared to cryoablation that only eliminated up to 66%. Importantly, NPS permanently eliminated these lesions with no recurrence and with minimal dermal fibrosis, underlying muscle atrophy, permanent hair follicle loss or other markers of permanent skin damage.
These findings suggest that NPS is a promising new modality for the clearance of melanoma tumors and is a more efficacious, less damaging approach than cryoablative methods for the treatment of aggressive malignant tumors.
纳米脉冲刺激疗法（NPS™）是一种新型生物电疗法，它通过施加超短电脉冲来触发被治疗组织中受调控的细胞死亡。NPS疗法并非通过加热或冷冻引发坏死，而是使细胞内细胞器通透化，以激活细胞自身的程序性或受调控细胞死亡的自我毁灭途径。与既能损伤结构组织又能扩散到病变边缘以外周边区域的冷冻疗法不同，NPS仅影响治疗区域内的细胞，而使周围组织和无细胞成分不受影响。
我们通过皮内注射B16-F10细胞在小鼠体内生成黑色素瘤肿瘤，并比较了纳米脉冲刺激疗法与冷冻消融在清除这些肿瘤方面的疗效及所致的皮肤损伤。
研究结果表明，NPS在清除B16-F10黑色素瘤病变方面更具优势。与冷冻消融仅能清除高达66%的肿瘤病变相比，NPS单次治疗能永久清除高达91%的所有肿瘤病变。重要的是，NPS能永久清除这些病变，无复发，且真皮纤维化、潜在肌肉萎缩、永久性毛囊丧失或其他永久性皮肤损伤标志物极少。
这些发现表明，NPS是清除黑色素瘤肿瘤的一种有前景的新方法，并且对于侵袭性恶性肿瘤的治疗而言，它是一种比冷冻消融方法更有效、损伤更小的治疗方式。
