Meng Kun, Eldar-Liebreich Michal, Nawade Bhagwat, Yahyaa Mosaab, Shaltiel-Harpaz Liora, Coll Moshe, Sadeh Asaf, Ibdah Mwafaq
Plant Sciences Institute, Newe Yaar Research Center, Agricultural Research Organization (ARO), Ramat Yishay, 30095 Israel.
Present Address: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
3 Biotech. 2023 Mar;13(3):94. doi: 10.1007/s13205-023-03511-4. Epub 2023 Feb 22.
Plant apocarotenoids have been shown to have a diverse biological role in herbivore-plant interactions. Despite their importance, little is known about herbivores' effect on apocarotenoid emissions in . In this study, we examined changes in apocarotenoid emissions in lettuce leaves after infestation by two insects, viz., larvae and aphids. We found that -ionone and -cyclocitral showed higher concentrations than the other apocarotenoids, with a significant increase as per the intensity of infestation of both herbivore species. Furthermore, we performed functional characterization of 1 () genes. Three genes were overexpressed in strains, and recombinant proteins were assayed for cleavage activity on an array of carotenoid substrates. The LsCCD1 protein cleaved carotene at the 9,10 (9',10') positions producing -ionone. The transcript analysis of genes revealed differential expression patterns under varying levels of herbivores' infestation, but the results were inconsistent with the pattern of -ionone concentrations. Our results suggest that LsCCD1 is involved in the production of -ionone, but other regulatory factors might be involved in its induction in response to herbivory. These results provide new insights into apocarotenoid production in response to insect herbivory in lettuce.
The online version contains supplementary material available at 10.1007/s13205-023-03511-4.
植物类胡萝卜素氧化产物已被证明在植食性动物与植物的相互作用中具有多种生物学作用。尽管它们很重要，但关于植食性动物对类胡萝卜素氧化产物排放的影响却知之甚少。在本研究中，我们检测了两种昆虫（即幼虫和蚜虫）侵染后生菜叶片中类胡萝卜素氧化产物排放的变化。我们发现，β-紫罗兰酮和β-环柠檬醛的浓度高于其他类胡萝卜素氧化产物，且随着两种植食性动物物种侵染强度的增加而显著增加。此外，我们对1个（）基因进行了功能表征。三个基因在菌株中过表达，并对重组蛋白在一系列类胡萝卜素底物上的裂解活性进行了测定。LsCCD1蛋白在9,10（9',10'）位置切割胡萝卜素，产生β-紫罗兰酮。基因的转录分析揭示了在不同程度的植食性动物侵染下的差异表达模式，但结果与β-紫罗兰酮浓度模式不一致。我们的结果表明，LsCCD1参与了β-紫罗兰酮的产生，但其他调节因子可能参与了其对食草作用的诱导。这些结果为生菜对昆虫食草作用响应中类胡萝卜素氧化产物的产生提供了新的见解。
在线版本包含可在10.1007/s13205-023-03511-4获取的补充材料。
