Van Goor Angelica, Pasternak Alex, Walugembe Muhammed, Chehab Nadya, Hamonic Glenn, Dekkers Jack C M, Harding John C S, Lunney Joan K
Animal Parasitic Diseases Laboratory, United States Department of Agriculture, Agricultural Research Services, Beltsville Agricultural Research Center, Beltsville, MD, United States.
Department of Animal Science, Purdue University, West Lafayette, IN, United States.
Front Genet. 2023 Feb 9;14:1110463. doi: 10.3389/fgene.2023.1110463. eCollection 2023.
Porcine reproductive and respiratory syndrome virus (PRRSV) causes respiratory disease in piglets and reproductive disease in sows. Piglet and fetal serum thyroid hormone (i.e., T3 and T4) levels decrease rapidly in response to Porcine reproductive and respiratory syndrome virus infection. However, the genetic control of T3 and T4 levels during infection is not completely understood. Our objective was to estimate genetic parameters and identify quantitative trait loci (QTL) for absolute T3 and/or T4 levels of piglets and fetuses challenged with Porcine reproductive and respiratory syndrome virus. Sera from 5-week-old pigs (N = 1792) at 11 days post inoculation (DPI) with Porcine reproductive and respiratory syndrome virus were assayed for T3 levels (piglet_T3). Sera from fetuses (N = 1,267) at 12 or 21 days post maternal inoculation (DPMI) with Porcine reproductive and respiratory syndrome virus of sows (N = 145) in late gestation were assayed for T3 (fetal_T3) and T4 (fetal_T4) levels. Animals were genotyped using 60 K Illumina or 650 K Affymetrix single nucleotide polymorphism (SNP) panels. Heritabilities, phenotypic correlations, and genetic correlations were estimated using ASREML; genome wide association studies were performed for each trait separately using Julia for Whole-genome Analysis Software (JWAS). All three traits were low to moderately heritable (10%-16%). Phenotypic and genetic correlations of piglet_T3 levels with weight gain (0-42 DPI) were 0.26 ± 0.03 and 0.67 ± 0.14, respectively. Nine significant quantitative trait loci were identified for piglet_T3, on chromosomes (SSC) 3, 4, 5, 6, 7, 14, 15, and 17, and collectively explaining 30% of the genetic variation (GV), with the largest quantitative trait loci identified on SSC5, explaining 15% of the genetic variation. Three significant quantitative trait loci were identified for fetal_T3 on SSC1 and SSC4, which collectively explained 10% of the genetic variation. Five significant quantitative trait loci were identified for fetal_T4 on SSC1, 6, 10, 13, and 15, which collectively explained 14% of the genetic variation. Several putative immune-related candidate genes were identified, including , , and . Thyroid hormone levels following Porcine reproductive and respiratory syndrome virus infection were heritable and had positive genetic correlations with growth rate. Multiple quantitative trait loci with moderate effects were identified for T3 and T4 levels during challenge with Porcine reproductive and respiratory syndrome virus and candidate genes were identified, including several immune-related genes. These results advance our understanding of growth effects of both piglet and fetal response to Porcine reproductive and respiratory syndrome virus infection, revealing factors associated with genomic control of host resilience.
猪繁殖与呼吸综合征病毒（PRRSV）可导致仔猪呼吸道疾病和母猪繁殖疾病。感染猪繁殖与呼吸综合征病毒后，仔猪和胎儿血清甲状腺激素（即T3和T4）水平会迅速下降。然而，感染期间T3和T4水平的遗传控制尚未完全明确。我们的目标是估计仔猪和受猪繁殖与呼吸综合征病毒攻击的胎儿绝对T3和/或T4水平的遗传参数，并鉴定数量性状位点（QTL）。对接种猪繁殖与呼吸综合征病毒11天后的5周龄猪（N = 1792）的血清进行T3水平检测（仔猪_T3）。对妊娠后期母猪（N = 145）接种猪繁殖与呼吸综合征病毒后12或21天的胎儿（N = 1267）血清进行T3（胎儿_T3）和T4（胎儿_T4）水平检测。使用60K Illumina或650K Affymetrix单核苷酸多态性（SNP）芯片对动物进行基因分型。使用ASREML估计遗传力、表型相关性和遗传相关性；使用全基因组分析软件Julia（JWAS）分别对每个性状进行全基因组关联研究。所有三个性状的遗传力均为低到中等（10%-16%）。仔猪_T3水平与体重增加（0-42天）的表型和遗传相关性分别为0.26±0.03和0.67±0.14。在第3、4、5、6、7、14、15和17号染色体（SSC）上鉴定出9个与仔猪_T3显著相关的数量性状位点，共解释了30%的遗传变异（GV），其中在SSC5上鉴定出的最大数量性状位点解释了15%的遗传变异。在SSC1和SSC4上鉴定出3个与胎儿_T3显著相关的数量性状位点，共解释了10%的遗传变异。在SSC1、6、10、13和15上鉴定出5个与胎儿_T4显著相关的数量性状位点，共解释了14%的遗传变异。鉴定出了几个假定的免疫相关候选基因，包括 、 和 。猪繁殖与呼吸综合征病毒感染后的甲状腺激素水平具有遗传性，且与生长速率呈正遗传相关性。在猪繁殖与呼吸综合征病毒攻击期间，鉴定出了多个对T3和T4水平有中等影响的数量性状位点，并鉴定出了候选基因，包括几个免疫相关基因。这些结果增进了我们对仔猪和胎儿对猪繁殖与呼吸综合征病毒感染反应的生长效应的理解，揭示了与宿主恢复力基因组控制相关的因素。
