Rodriguez Morales David, Ilieva Mirolyuba, Rennie Sarah, Uchida Shizuka
Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Department of Clinical Medicine, Center for RNA Medicine, Aalborg University, Copenhagen, Denmark.
Am J Physiol Cell Physiol. 2023 Apr 1;324(4):C837-C842. doi: 10.1152/ajpcell.00024.2023. Epub 2023 Feb 27.
Adenosine to inosine (A-to-I) RNA editing is one of the most frequent RNA modifications found in the mammalian transcriptome. Recent studies clearly indicate that RNA editing enzymes, adenosine deaminase acting on RNAs (ADARs), are upregulated in stressed cells and under disease conditions, suggesting that monitoring RNA editing patterns might be useful as diagnostic biomarkers of various diseases. Here, we provide an overview of epitranscriptomics, and focus particularly on the detection and analysis of A-to-I RNA editing using bioinformatic tools in RNA-seq data sets, as well as briefly reviewing the existing evidence about its involvement in disease progressions. Finally, we argue for the detection of RNA editing patterns as part of the routine analysis in RNA-based data sets, with the aim of accelerating the identification of RNA editing targets linked to disease.
腺苷到肌苷（A-to-I）RNA编辑是哺乳动物转录组中最常见的RNA修饰之一。最近的研究清楚地表明，RNA编辑酶，即作用于RNA的腺苷脱氨酶（ADARs），在应激细胞和疾病状态下会上调，这表明监测RNA编辑模式可能作为各种疾病的诊断生物标志物。在这里，我们概述了表观转录组学，特别关注使用生物信息学工具在RNA测序数据集中检测和分析A-to-I RNA编辑，以及简要回顾关于其参与疾病进展的现有证据。最后，我们主张将RNA编辑模式的检测作为基于RNA的数据集常规分析的一部分，目的是加速鉴定与疾病相关的RNA编辑靶点。
