Pharmacy Faculty, Hubei University of Chinese Medicine, Wuhan 430000, P. R.China.
Anal Methods. 2023 Mar 9;15(10):1378-1386. doi: 10.1039/d2ay01972h.
Electrochemical techniques have been applied to determine quercetin and to evaluate its antioxidant effect. Deep eutectic solvents as a new generation of green solvents are promising electrolyte catalytically active additives for electrochemical oxidation of quercetin. Herein, in this work, Au was directly electrodeposited on the surface of graphene-modified glassy carbon electrodes, and AuNPs/GR/GC electrodes were fabricated. Choline chloride-based ionic liquids as deep eutectic solvents were readily prepared and applied to detect quercetin in buffer solutions, allowing a detection enhancement. X-ray diffraction and scanning electron microscopy were carried out to characterize the morphology of AuNPs/GR/GCE. Fourier transform infrared spectroscopy was carried out to interpret the H-bond interactions between the DES and quercetin. This electrochemical sensor exhibited good analytical performance. The low detection limit was reduced to 0.5 μM in a 15% DES solution, which was 300% higher than the signal without DES. It proved to be fast and environmentally friendly for the determination of quercetin and the DES had no effect on antioxidant effects of quercetin. In addition, it has been successfully applied in real sample analysis.
电化学技术已被用于测定槲皮素并评估其抗氧化作用。深共晶溶剂作为新一代绿色溶剂，是一种很有前途的电解质，可作为电化学氧化槲皮素的催化活性添加剂。在此，在这项工作中，金被直接电沉积在石墨烯修饰的玻碳电极表面，制备了 AuNPs/GR/GC 电极。基于氯化胆碱的离子液体作为深共晶溶剂很容易制备，并应用于缓冲溶液中检测槲皮素，提高了检测能力。通过 X 射线衍射和扫描电子显微镜对 AuNPs/GR/GCE 的形貌进行了表征。傅里叶变换红外光谱用于解释 DES 和槲皮素之间的氢键相互作用。这种电化学传感器表现出良好的分析性能。在 15%的 DES 溶液中，检测限降低到 0.5 μM，比没有 DES 的信号高 300%。它被证明是快速和环保的，用于测定槲皮素，而且 DES 对槲皮素的抗氧化作用没有影响。此外，它已成功应用于实际样品分析。
