Sater Mai S, AlDehaini Dhuha M B, Malalla Zainab Hasan Abdulla, Ali Muhalab E, Giha Hayder Ahmed
Department of Medical Biochemistry, College of Medicine and Medical Sciences (CMMS), Arabian Gulf University (AGU), Manama, Kingdom of Bahrain.
Kuwait Medical Genetic Center, Kuwait City, Kuwait.
Horm Mol Biol Clin Investig. 2023 Feb 28;44(3):259-269. doi: 10.1515/hmbci-2022-0103. eCollection 2023 Sep 1.
Type 2 diabetes (T2D) is known to be associated with chronic inflammation, but the inflammatory regulators/markers are not exactly defined and the link between them remains undetermined. The objective of this study is to identify these markers by testing traditional (IL6 & IL8) and non-traditional (TREM1 & uPAR) inflammatory markers.
Data and blood samples were obtained from 114 T2D and 74 non-diabetic Kuwaiti subjects attending health facilities in Kuwait. Chemical analyzers were used to measure glycemic and lipid profiles, while ELISA was used to measure plasma levels of insulin and several inflammatory markers.
Showed that the IL-6 and TREM1 were significantly higher in T2D compared to non-diabetic controls, and the uPAR level was borderline higher in T2D but significantly correlated with IL-6 levels. Unexpectedly, IL8 was significantly below normal in T2D and IL6/IL8 ratio was significantly higher in T2D patients. Unlike other tested markers, uPAR was in addition strongly correlated with insulin levels and HOMA-IR index.
Raised levels of IL6, TREMI, IL6/IL8 ratio, and the strong positive correlation of plasma levels of uPAR with IL-6, insulin, and HOMA-IR index, are reliable spectators of chronic inflammation in T2D patients. The reduced level of IL-8 in T2D was a peculiar observation that needs further explanation. Finally, the consequences and impact of the sustained rise of these inflammatory regulators in diabetic tissues need to be meticulously explored.
2型糖尿病（T2D）已知与慢性炎症相关，但炎症调节因子/标志物尚未完全明确，它们之间的联系也未确定。本研究的目的是通过检测传统（IL6和IL8）和非传统（TREM1和uPAR）炎症标志物来识别这些标志物。
从科威特的114名2型糖尿病患者和74名非糖尿病科威特受试者中获取数据和血样，这些受试者均前往科威特的医疗机构就诊。使用化学分析仪测量血糖和血脂水平，同时使用酶联免疫吸附测定法（ELISA）测量血浆胰岛素水平和几种炎症标志物。
显示与非糖尿病对照组相比，2型糖尿病患者的IL-6和TREM1显著更高，2型糖尿病患者的uPAR水平略高但与IL-6水平显著相关。出乎意料的是，2型糖尿病患者的IL8显著低于正常水平，且2型糖尿病患者的IL6/IL8比值显著更高。与其他检测标志物不同，uPAR还与胰岛素水平和HOMA-IR指数密切相关。
IL6、TREMI水平升高、IL6/IL8比值升高以及uPAR血浆水平与IL-6、胰岛素和HOMA-IR指数的强正相关，是2型糖尿病患者慢性炎症的可靠指标。2型糖尿病患者中IL-8水平降低是一个需要进一步解释的特殊观察结果。最后，需要仔细探讨这些炎症调节因子在糖尿病组织中持续升高的后果和影响。
