Center for Flexible Electronics Technology, Tsinghua University, No. 30 Shuangqing Road, Beijing, 100084, P. R. China.
MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering & Key Laboratory of Micro-systems and Micro-structures, Ministry of Education, Harbin Institute of Technology, No. 92 Xidazhi Street, Harbin, 150001, P. R. China.
Adv Mater. 2023 May;35(20):e2210948. doi: 10.1002/adma.202210948. Epub 2023 Mar 29.
Luminescent monitoring of endogenous hydrogen peroxide (H O ) in tumors is conducive to understanding metastasis and developing novel therapeutics. The clinical transformation is obstructed by the limited light penetration depth, toxicity of nano-probes, and lack of long-term monitoring modes of up to days or months. New monitoring modes are introduced via specific probes and implantable devices, which can achieve real-time monitoring with a readout frequency of 0.01 s or long-term monitoring for months to years. Near-infrared dye-sensitized upconversion nanoparticles (UCNPs) are fabricated as the luminescent probes, and the specificity to reactive oxygen species is subtly regulated by the self-assembled monolayers on the surfaces of UCNPs. Combined with the passive implanted system, a 20-day monitoring of H O in the rat model of ovarian cancer with peritoneal metastasis is achieved, in which the limited light penetration depth and toxicity of nano-probes are circumvented. The developed monitoring modes show great potential in accelerating the clinical transformation of nano-probes and biochemical detection methods.
内源性过氧化氢 (H2O2) 的荧光监测有助于了解转移并开发新的治疗方法。但由于光穿透深度有限、纳米探针的毒性以及缺乏长达数天或数月的长期监测模式，其临床转化受到了阻碍。通过特定的探针和植入式设备引入了新的监测模式，可实现 0.01 秒的读取频率的实时监测或长达数月至数年的长期监测。近红外染料敏化上转换纳米粒子 (UCNPs) 被制成荧光探针，通过 UCNPs 表面的自组装单分子层来微妙调节其对活性氧的特异性。结合被动植入系统，对具有腹膜转移的卵巢癌大鼠模型中的 H2O2 进行了 20 天的监测，从而避免了纳米探针的有限光穿透深度和毒性。所开发的监测模式在加速纳米探针和生化检测方法的临床转化方面具有巨大潜力。
