促卵泡激素刺激和年龄对未成年母猪卵巢和子宫特征及组织形态计量学的影响。

The impact of FSH stimulation and age on the ovarian and uterine traits and histomorphometry of prepubertal gilts.

机构信息

Departamento de Veterinária, Universidade Federal de Viçosa, Av Peter Henry Rolfs, Campus Universitário, CEP 36570-900, Viçosa, MG, Brazil.

出版信息

Domest Anim Endocrinol. 2023 Apr;83:106786. doi: 10.1016/j.domaniend.2023.106786. Epub 2023 Jan 27.

DOI:10.1016/j.domaniend.2023.106786

PMID:36848729

Abstract

This study investigated the effect of age and follicle stimulating hormone (FSH) treatment on the estradiol (E2) plasma concentration, ovarian follicle development, endometrial histomorphometry, and ultrasonographic parameters of the ovaries and uterus in prepubertal gilts. Thirty-five prepubertal gilts were grouped according to age (140 or 160 d), and within each age, gilts were allotted to receive 100 mg of FSH (treated; G140 + FSH [n = 10] and G160 + FSH [n = 7]) or saline solution (control; G140 + control [n = 10] and G160 + control [n = 8]). The total dose of FSH was divided into 6 similar doses administered every 8 h (days 0-2). Before and after FSH treatment, blood sample was collected, and transabdominal scanning of the ovaries and uterus was performed. Twenty-four hours after the last FSH injection, the gilts were slaughtered and their ovaries and uterus were processed for histological and histomorphometric analysis. The histomorphometric parameters of the uterus differed (P < 0.05) between prepubertal gilts at 160 d and 140 d of age. Moreover, changes (P < 0.05) in uterine and ovarian ultrasound images occurred between 140 and 160 d of age. Age and FSH treatment did not affect (P > 0.05) E2 plasma concentrations. Follicle stimulating hormone treatment did not affect (P > 0.05) the early stage of folliculogenesis in the prepubertal gilts; however, the number of early atretic follicles decreased (P < 0.05) after the FSH treatment. Follicle stimulating hormone administration increased (P < 0.05) the number of medium follicles and decreased (P < 0.05) the number of small follicles in 140 and 160 d old gilts. In the endometrium, luminal/glandular epithelium height and glandular diameter increased (P < 0.05) after FSH treatment. Thus, injections of 100 mg of FSH stimulate the endometrium epithelium and induce follicular growth to a medium follicle size without affecting the preantral stages in prepubertal gilts; also, the uterine macroscopic morphometry does not change from 140 to 160 d of age.

摘要

这项研究旨在探讨年龄和卵泡刺激素（FSH）治疗对未成年小母猪的雌二醇（E2）血浆浓度、卵巢卵泡发育、子宫内膜组织形态计量学以及卵巢和子宫的超声参数的影响。将 35 头未成年小母猪根据年龄（140 或 160 日龄）分组，在每个年龄组内，小母猪被分配接受 100mg 的 FSH（处理组；G140+FSH[n=10]和 G160+FSH[n=7]）或生理盐水溶液（对照组；G140+对照[n=10]和 G160+对照[n=8]）。FSH 的总剂量分为 6 个相似的剂量，每 8 小时给药一次（第 0-2 天）。在 FSH 治疗前后采集血液样本，并进行经腹卵巢和子宫扫描。最后一次 FSH 注射后 24 小时，屠宰小母猪，并对其卵巢和子宫进行组织学和组织形态计量学分析。160 日龄和 140 日龄未成年小母猪的子宫组织形态计量学参数存在差异（P<0.05）。此外，140 日龄至 160 日龄之间，子宫和卵巢的超声图像发生了变化（P<0.05）。年龄和 FSH 治疗对 E2 血浆浓度没有影响（P>0.05）。FSH 处理对未成年小母猪的卵泡发生早期阶段没有影响（P>0.05）；然而，FSH 处理后早期闭锁卵泡的数量减少（P<0.05）。FSH 给药增加了 140 日龄和 160 日龄小母猪中型卵泡的数量，并减少了小型卵泡的数量（P<0.05）。在子宫内膜中，FSH 处理后管腔/腺体上皮高度和腺体直径增加（P<0.05）。因此，100mg 的 FSH 注射可刺激未成年小母猪的子宫内膜上皮，并诱导卵泡生长至中型卵泡大小，而不影响未成年小母猪的窦前阶段；此外，140 日龄至 160 日龄时，子宫的宏观形态计量学没有变化。