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改良的2-硫代巴比妥酸法测定家禽脂质氧化

Modified extraction 2-thiobarbituric acid method for measuring lipid oxidation in poultry.

作者信息

Salih A M, Smith D M, Price J F, Dawson L E

机构信息

Department of Food Science & Human Nutrition, Michigan State University, East Lansing 48824.

出版信息

Poult Sci. 1987 Sep;66(9):1483-8. doi: 10.3382/ps.0661483.

DOI:10.3382/ps.0661483
PMID:3684874
Abstract

A simple and accurate objective test for monitoring lipid oxidation in poultry products is needed. Thiobarbituric acid (TBA) values of cooked poultry meat were determined by a distillation method and by a modified extraction method. Perchloric acid was used in the extraction method to release TBA reactive substances from the meat. The maximum absorption wavelength of the TBA-malonaldehyde complex was 531 nm. The TBA values for cooked chicken and turkey were highly correlated (r = .91) for both the extraction and distillation methods. Sensory scores of warmed-over flavor in precooked poultry were highly correlated with TBA values in both the distillation (r = .83) and extraction (r = .85) methods. Results indicate that the extraction method is faster, easier to perform, and as accurate as the distillation method for monitoring lipid oxidation in poultry.

摘要

需要一种简单且准确的客观测试方法来监测禽肉产品中的脂质氧化。采用蒸馏法和改良提取法测定了熟禽肉的硫代巴比妥酸(TBA)值。提取法中使用高氯酸从肉中释放TBA反应性物质。TBA-丙二醛复合物的最大吸收波长为531nm。对于提取法和蒸馏法,熟鸡肉和火鸡肉的TBA值高度相关(r = 0.91)。预煮禽肉中加热过度风味的感官评分与蒸馏法(r = 0.83)和提取法(r = 0.85)中的TBA值高度相关。结果表明,在监测禽肉脂质氧化方面,提取法更快、更易于操作,且与蒸馏法一样准确。

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