Motta Giulia, Gualtieri Maurizio, Saibene Melissa, Bengalli Rossella, Brigliadori Andrea, Carrière Marie, Mantecca Paride
Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
Research Centre POLARIS, Department of Earth and Environmental Sciences, University of Milano-Bicocca, 20126 Milan, Italy.
Toxics. 2023 Feb 20;11(2):195. doi: 10.3390/toxics11020195.
Silver nanoparticles (Ag NPs) are among the most widely used metal-based nanomaterials (NMs) and their applications in different products, also as antibacterial additives, are increasing. In the present manuscript, according to an adverse outcome pathway (AOP) approach, we tested two safe-by-design (SbD) newly developed Ag NPs coated with hydroxyethyl cellulose (HEC), namely AgHEC powder and AgHEC solution. These novel Ag NPs were compared to two reference Ag NPs (naked and coated with polyvinylpyrrolidone-PVP). Cell viability, inflammatory response, reactive oxygen species, oxidative DNA damage, cell cycle, and cell-particle interactions were analyzed in the alveolar in vitro model, A549 cells. The results show a different toxicity pattern of the novel Ag NPs compared to reference NPs and that between the two novel NPs, the AgHEC solution is the one with the lower toxicity and to be further developed within the SbD framework.
银纳米颗粒（Ag NPs）是应用最为广泛的金属基纳米材料（NMs）之一，其在不同产品中的应用，包括作为抗菌添加剂，正在不断增加。在本手稿中，根据不良结局途径（AOP）方法，我们测试了两种新开发的通过设计确保安全（SbD）的、涂有羟乙基纤维素（HEC）的Ag NPs，即AgHEC粉末和AgHEC溶液。将这些新型Ag NPs与两种参比Ag NPs（裸Ag NPs和涂有聚乙烯吡咯烷酮 - PVP的Ag NPs）进行比较。在体外肺泡模型A549细胞中分析了细胞活力、炎症反应、活性氧、氧化性DNA损伤、细胞周期和细胞 - 颗粒相互作用。结果表明，与参比纳米颗粒相比，新型Ag NPs具有不同的毒性模式，并且在两种新型纳米颗粒之间，AgHEC溶液毒性较低，将在SbD框架内进一步开发。
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