Microbial Biotechnologies, Agrosciences and Environment Laboratory (BioMAgE), Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh 40000, Morocco.
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini", 42124 Brescia, Italy.
Viruses. 2023 Feb 2;15(2):422. doi: 10.3390/v15020422.
The number of newly described sandfly-borne phleboviruses has been steadily growing in recent years. Some phleboviruses are human pathogens, but their health relevance is largely uncharacterized. We aimed to investigate the circulation of these viruses in the Emilia-Romagna region where several have already been described. A total of 482 sandflies were collected in a site in Reggio Emilia in 2019 and 2020. Sandflies collected in 2020 were grouped in 21 pools with a maximum of 25 sandflies per pool, submitted to real time PCR, and isolated in Vero cell culture. Complete genome sequencing showed the isolation of a strain of a Ponticelli III virus. This virus, which belongs to the species , differed in the M segment from the Ponticelli I and Ponticelli II viruses. Analysis performed on the genomic segments of the newly isolated virus compared with other phleboviruses highlighted a strong purifying selection in the L segments, and different substitution saturation, highest in the M segments. Future research should address the ecological processes driving the occurrence of these novel phleboviruses and their possible impact on public health.
近年来，新描述的沙蝇传播黄病毒的数量一直在稳步增长。一些黄病毒是人类病原体，但它们的健康相关性在很大程度上尚未确定。我们旨在研究这些病毒在已经有几种病毒被描述的艾米利亚-罗马涅地区的传播情况。2019 年和 2020 年，在雷焦艾米利亚的一个地点共收集了 482 只沙蝇。2020 年收集的沙蝇被分为 21 个组，每组最多有 25 只沙蝇，进行实时 PCR，并在 Vero 细胞培养中分离。全基因组测序显示分离出一株波蒂切利 III 病毒的菌株。该病毒属于 种，与波蒂切利 I 和波蒂切利 II 病毒在 M 片段上有所不同。对新分离病毒的基因组片段与其他黄病毒进行的分析表明，L 片段存在强烈的纯化选择，而 M 片段的替代饱和程度最高。未来的研究应解决导致这些新型黄病毒出现的生态过程及其对公共卫生的可能影响。
