- 诱导的 HSU 细胞中的抗病毒 RNAi 反应。

Antiviral RNAi Response in -Derived HSU Cells.

机构信息

Bernhard-Nocht-Institute for Tropical Medicine, 20359 Hamburg, Germany.

German Center for Infection Research, Partner Site Hamburg-Lübeck-Borstel-Riems, 20359 Hamburg, Germany.

出版信息

Viruses. 2023 Feb 4;15(2):436. doi: 10.3390/v15020436.

DOI:10.3390/v15020436

PMID:36851650

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9968050/

Abstract

spp. mosquitoes are important vectors of viruses, such as West Nile virus, Eastern equine encephalitis virus and Rift valley fever virus. However, their interactions with innate antiviral immunity, especially RNA interference (RNAi), are not well known. Most research on RNAi pathways in mosquitoes is focused on the tropical vector mosquito . Here, we investigated the production of arbovirus-specific small RNAs in -derived HSU cells. Furthermore, by silencing RNAi-related proteins, we investigated the antiviral role of these proteins for two different arboviruses: Semliki Forest virus (SFV) and Bunyamwera orthobunyavirus (BUNV). Our results showed an expansion of Ago2 and Piwi6 in compared to . While silencing Ago2a and Ago2b increased BUNV replication, only Ago2b showed antiviral activity against SFV. Our results suggest differences in the function of and RNAi proteins and highlight the virus-specific function of these proteins in .

摘要

种属的蚊子是病毒的重要载体，例如西尼罗河病毒、东部马脑炎病毒和裂谷热病毒。然而，它们与先天抗病毒免疫的相互作用，特别是 RNA 干扰（RNAi），尚不清楚。蚊子中 RNAi 途径的大多数研究都集中在热带病媒蚊子上。在这里，我们研究了在 -衍生的 HSU 细胞中产生虫媒病毒特异性小 RNA。此外，通过沉默 RNAi 相关蛋白，我们研究了这些蛋白对两种不同虫媒病毒：辛德毕斯病毒（SFV）和邦巴韦拉 orthobunyavirus（BUNV）的抗病毒作用。我们的结果表明，与相比，Ago2 和 Piwi6 在中扩张。沉默 Ago2a 和 Ago2b 会增加 BUNV 的复制，而只有 Ago2b 对 SFV 表现出抗病毒活性。我们的结果表明和 RNAi 蛋白的功能存在差异，并强调了这些蛋白在中的病毒特异性功能。