介形虫病毒和内盖夫病毒与虫媒病毒的相互作用及 Culex tarsalis 细胞中的 RNAi 反应。

Interaction of Mesonivirus and Negevirus with arboviruses and the RNAi response in Culex tarsalis-derived cells.

机构信息

Bernhard-Nocht-Institute for Tropical Medicine, 20359, Hamburg, Germany.

School of Basic and Biomedical Sciences, Department of Biomedical Sciences, University of Health and Allied Sciences, PMB 31, Ho, Ghana.

出版信息

Parasit Vectors. 2023 Oct 13;16(1):361. doi: 10.1186/s13071-023-05985-w.

DOI:10.1186/s13071-023-05985-w

PMID:37833743

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10576325/

Abstract

BACKGROUND

Mosquito-specific viruses (MSVs) comprise a variety of different virus families, some of which are known to interfere with infections of medically important arboviruses. Viruses belonging to the family Mesoniviridae or taxon Negevirus harbor several insect-specific viruses, including MSVs, which are known for their wide geographical distribution and extensive host ranges. Although these viruses are regularly identified in mosquitoes all over the world, their presence in mosquitoes in Germany had not yet been reported.

METHODS

A mix of three MSVs (Yichang virus [Mesoniviridae] and two negeviruses [Daeseongdong virus and Dezidougou virus]) in a sample that contained a pool of Coquillettidia richiardii mosquitoes collected in Germany was used to investigate the interaction of these viruses with different arboviruses in Culex-derived cells. In addition, small RNA sequencing and analysis of different mosquito-derived cells infected with this MSV mix were performed.

RESULTS

A strain of Yichang virus (Mesoniviridae) and two negeviruses (Daeseongdong virus and Dezidougou virus) were identified in the Cq. richiardii mosquitoes sampled in Germany, expanding current knowledge of their circulation in central Europe. Infection of mosquito-derived cells with these three viruses revealed that they are targeted by the small interfering RNA (siRNA) pathway. In Culex-derived cells, co-infection by these three viruses had varying effects on the representative arboviruses from different virus families (Togaviridae: Semliki forest virus [SFV]; Bunyavirales: Bunyamwera orthobunyavirus [BUNV]; or Flaviviridae: Usutu virus [USUV]). Specifically, persistent MSV co-infection inhibited BUNV infection, as well as USUV infection (but the latter only at specific time points). However, the impact on SFV infection was only noticeable at low multiplicity of infection (MOI 0.1) and at specific time points in combination with the infection status.

CONCLUSIONS

Taken together, these results are important findings that will lead to a better understanding of the complex interactions of MSVs, mosquitoes and arboviruses.

摘要

背景

蚊特异性病毒（MSV）包含多种不同的病毒科，其中一些已知会干扰医学上重要的虫媒病毒感染。属于 Mesoniviridae 科或 Negevirus 分类群的病毒携带几种昆虫特异性病毒，包括 MSV，其分布广泛，宿主范围广泛。尽管这些病毒在世界各地的蚊子中经常被发现，但在德国的蚊子中尚未报告其存在。

方法

在含有德国采集的 Coquillettidia richiardii 蚊子混合池中，使用三种 MSV（Yichang 病毒[Mesoniviridae]和两种 Negeviruses[Daeseongdong 病毒和 Dezidougou 病毒]）的混合物来研究这些病毒与 Culex 衍生细胞中不同虫媒病毒的相互作用。此外，还对感染这种 MSV 混合物的不同蚊子衍生细胞进行了小 RNA 测序和分析。

结果

在德国采集的 Cq. richiardii 蚊子中鉴定出一种 Yichang 病毒（Mesoniviridae）株和两种 Negeviruses（Daeseongdong 病毒和 Dezidougou 病毒），扩大了它们在中欧传播的现有知识。用这三种病毒感染蚊子衍生细胞表明，它们是小干扰 RNA（siRNA）途径的靶标。在 Culex 衍生细胞中，这三种病毒的共同感染对来自不同病毒科的代表虫媒病毒（披膜病毒科：Semliki 森林病毒[SFV]； Bunyavirales：Bunyamwera 正粘病毒[BUNV]；或黄病毒科：乌苏图病毒[USUV]）有不同的影响。具体而言，持续的 MSV 共同感染抑制了 BUNV 感染以及 USUV 感染（但后者仅在特定时间点）。然而，对 SFV 感染的影响仅在低感染复数（MOI 0.1）和特定时间点与感染状态结合时才明显。