寡核苷酸序列的化学合成：亚磷酰胺化学。
Chemical Synthesis of Oligonucelotide Sequences: Phosphoramidite Chemistry.
机构信息
Reading School of Pharmacy, University of Reading, Reading, UK.
出版信息
Methods Mol Biol. 2023;2633:185-193. doi: 10.1007/978-1-0716-3004-4_14.
PMID:36853465
Abstract
Oligonucleotides are used in a variety of molecular biology techniques, from next-generation sequencing to genetic testing. Maintaining the sequence fidelity of synthetic oligonucleotides is critical to their use. This chapter describes the steps of solid phase oligonucleotide synthesis and purification, which enables the synthesis of oligonucleotides with specific sequences and high purity.
摘要
寡核苷酸在各种分子生物学技术中都有应用，从下一代测序到基因检测。保持合成寡核苷酸的序列保真度对于它们的应用至关重要。本章描述了固相寡核苷酸合成和纯化的步骤，这使得具有特定序列和高纯度的寡核苷酸的合成成为可能。
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