使用发光和显微镜对小鼠胃肿瘤类器官的活力和生长进行纵向定量。

Longitudinal quantification of mouse gastric tumor organoid viability and growth using luminescence and microscopy.

机构信息

Olivia Newton-John Cancer Research Institute and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.

出版信息

STAR Protoc. 2023 Mar 17;4(1):102110. doi: 10.1016/j.xpro.2023.102110. Epub 2023 Feb 10.

DOI:10.1016/j.xpro.2023.102110

PMID:36853665

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9947417/

Abstract

Tumor-derived organoids are valuable for testing anti-cancer drugs in vitro, but existing lysis-based protocols for viability measurement are laborious and restricted at a single time point. Here, we provide a lysis-free protocol for longitudinal and rapid assessment of mouse gastric tumor organoid viability and growth. We describe organoid plating, viability assessment via luminescence measurement, quantification of organoid growth by microscopy imaging, and treatment of organoids with test compounds to evaluate the effects on viability and growth at various time points.

摘要

肿瘤类器官在体外测试抗癌药物方面具有重要价值，但现有的基于裂解的活力测量方法既繁琐，又只能在单一时间点进行测量。在此，我们提供了一种无裂解的方案，用于对小鼠胃肿瘤类器官的活力和生长进行纵向和快速评估。我们描述了类器官的铺板、通过发光测量进行活力评估、通过显微镜成像对类器官生长进行定量以及用测试化合物处理类器官，以评估在不同时间点对活力和生长的影响。