通过亲和筛选鉴定识别肺炎球菌荚膜的小鼠枯否细胞受体。
Identification of the mouse Kupffer cell receptors recognizing pneumococcal capsules by affinity screening.
机构信息
Center for Infectious Disease Research, Department of Basic Medical Science, School of Medicine, Tsinghua University, Beijing, China.
Center for Infectious Disease Research, Department of Basic Medical Science, School of Medicine, Tsinghua University, Beijing, China; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
出版信息
STAR Protoc. 2023 Mar 17;4(1):102065. doi: 10.1016/j.xpro.2023.102065. Epub 2023 Jan 24.
Kupffer cells (KCs) are the major sentinels to guard the bloodstream by recognizing diverse microbial ligands of blood-borne pathogens. Here, we establish a protocol for identifying the KC receptors recognizing the capsular polysaccharides (CPSs) of low-virulence Streptococcus pneumoniae in a mouse model. This protocol includes preparation of CPS-coated microspheres and KC membrane proteins, affinity pulldown of CPS-binding proteins, and functional validation of the CPS receptors. This protocol provides a platform to investigate the receptor-ligand interactions between KCs and encapsulated bacteria. For complete details on the use and execution of this protocol, please refer to An et al. (2022)..
枯否细胞（KCs）是通过识别血液病原体的各种微生物配体来保护血液的主要哨兵。在这里，我们建立了一个在小鼠模型中鉴定识别低毒力肺炎链球菌荚膜多糖（CPS）的 KC 受体的方案。该方案包括 CPS 包被微球和 KC 膜蛋白的制备、CPS 结合蛋白的亲和下拉以及 CPS 受体的功能验证。该方案为研究 KC 和囊封细菌之间的受体-配体相互作用提供了一个平台。有关此方案的使用和执行的完整详细信息，请参阅 An 等人。(2022)。
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