Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
STAR Protoc. 2023 Mar 17;4(1):102018. doi: 10.1016/j.xpro.2022.102018. Epub 2023 Jan 25.
Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer cells in the context of the lung fibrogenic microenvironment. Utilizing the cell transfer technique, we detail a basis for therapeutic approaches in pulmonary fibrosis and tools for precision medicine against lung cancer. For complete details on the use and execution of this protocol, please refer to Miyata et al. (2022)..
肺纤维化是一种以上皮损伤和成纤维细胞激活为特征的过程。它也被公认为肺癌的易患因素。在这里，我们提供了一个建立体内模型的方案，以评估肺泡上皮细胞 2 型和肺癌细胞在肺纤维化微环境中的动态变化。利用细胞转移技术，我们详细介绍了一种治疗肺纤维化的方法基础和针对肺癌的精准医疗工具。有关该方案使用和实施的完整详细信息，请参见 Miyata 等人。（2022）。
