Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA; email:
Department of Neurology, Vanderbilt University Medical Center, Vanderbilt University, Nashville, Tennessee, USA.
Annu Rev Anal Chem (Palo Alto Calif). 2023 Jun 14;16(1):379-400. doi: 10.1146/annurev-anchem-091622-092353. Epub 2023 Feb 28.
The identification of thousands of proteins and their relative levels of expression has furthered understanding of biological processes and disease and stimulated new systems biology hypotheses. Quantitative proteomics workflows that rely on analytical assays such as mass spectrometry have facilitated high-throughput measurements of proteins partially due to multiplexing. Multiplexing allows proteome differences across multiple samples to be measured simultaneously, resulting in more accurate quantitation, increased statistical robustness, reduced analysis times, and lower experimental costs. The number of samples that can be multiplexed has evolved from as few as two to more than 50, with studies involving more than 10 samples being denoted as enhanced multiplexing or hyperplexing. In this review, we give an update on emerging multiplexing proteomics techniques and highlight advantages and limitations for enhanced multiplexing strategies.
鉴定数千种蛋白质及其相对表达水平进一步加深了对生物过程和疾病的理解，并激发了新的系统生物学假设。依赖于分析检测（如质谱）的定量蛋白质组学工作流程促进了蛋白质的高通量测量，部分原因是多重化。多重化允许同时测量多个样本之间的蛋白质组差异，从而实现更准确的定量、更高的统计稳健性、缩短分析时间和降低实验成本。可进行多重化的样本数量已经从两个发展到 50 多个，涉及 10 多个样本的研究被称为增强型多重化或超多重化。在这篇综述中，我们介绍了新兴的多重化蛋白质组学技术，并强调了增强型多重化策略的优点和局限性。
