轻质碳酸钙可提高出芽短梗霉在高糖浓度下的普鲁兰多糖生物合成能力。
Light calcium carbonate improves pullulan biosynthesis by Aureobasidium pullulans under high concentration of sugar.
作者信息
Yang Jinyu, Li Xiwen, Zhao Shuangzhi, Yuan Wei, Zhou Qingxin, Zhang Yanhao, Qiu Jiying, Wang Junhua, Zhu Qingjun, Yang Xiaoyu, Jiang Xiaoxiao, Tian Chengsen, Chen Leilei
机构信息
Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Jinan 250100, China.
Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Jinan 250100, China; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
出版信息
Food Chem. 2023 Jul 30;415:135760. doi: 10.1016/j.foodchem.2023.135760. Epub 2023 Feb 21.
The effects of light calcium carbonate (CaCO) on pullulan biosynthesis by Aureobasidium pullulans NCPS2016 were investigated. Light CaCO enhanced pullulan production by 12.4 % when added to the low concentration of fructose broth compared with KHPO. Pullulan production was further improved when increasing both the concentrations of light CaCO and fructose. Compared to KHPO, light CaCO improved the activities of UDP-glucose pyrophosphorylase, α-phosphoglucose mutase, UDP-glucosyltransferase, and glucosyltransferase relevant to pullulan biosynthesis, and the gene transcriptional levels of UDP-glucose pyrophosphorylase, α-phosphoglucose mutase, UDP-glucosyltransferase, and glucose kinase were enhanced. During 30-liter fermentation, 144.3 g/L of purified pullulan was produced from 200 g/L of fructose and 15 g/L of light CaCO within 168 h, with the yield and productivity of 0.72 g/g and 0.86 g/L/h respectively. This is the first report that light CaCO improves pullulan production significantly.
研究了轻质碳酸钙（CaCO）对出芽短梗霉NCPS2016合成茁霉多糖的影响。与KHPO相比，当添加到低浓度果糖肉汤中时，轻质CaCO使茁霉多糖产量提高了12.4%。当同时提高轻质CaCO和果糖的浓度时，茁霉多糖产量进一步提高。与KHPO相比，轻质CaCO提高了与茁霉多糖生物合成相关的UDP-葡萄糖焦磷酸化酶、α-磷酸葡萄糖变位酶、UDP-葡萄糖基转移酶和葡萄糖基转移酶的活性，并且提高了UDP-葡萄糖焦磷酸化酶基因、α-磷酸葡萄糖变位酶基因、UDP-葡萄糖基转移酶基因和葡萄糖激酶基因的转录水平。在30升发酵过程中，在168小时内由200克/升果糖和15克/升轻质CaCO产生了144.3克/升的纯化茁霉多糖，产率和生产率分别为0.72克/克和0.86克/升/小时。这是首次报道轻质CaCO能显著提高茁霉多糖产量。
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