Pain Research and Intervention Center of Excellence (PRICE) at the University of Florida, Gainesville, Florida, USA,
Department of Community Dentistry and Behavioral Science, The University of Florida, Gainesville, Florida, USA,
Lifestyle Genom. 2023;16(1):98-105. doi: 10.1159/000529823. Epub 2023 Feb 28.
Recent evidence suggests that vitamin D may interact with the epigenome and play a role in the pain experience. In order for proper functioning to occur, there must be an adequate level of vitamin D present, made possible by enzymatic reactions that allow vitamin D to be biologically active. The purpose of this study was to explore the epigenetic landscape of genes involved in vitamin D metabolism in individuals with and without chronic knee pain.
Community-dwelling individuals recruited as part of a larger study focused on knee pain provided demographic, clinical, and pain-related information, as well as an intravenous blood sample to determine DNA methylation levels at CpG sites.
There were differences in DNA methylation between those with and without pain in genes that code for enzymes related to vitamin D metabolism: CYP27B1 (1-α-hydroxylase). There was also hypermethylation on the gene that codes for the vitamin D receptor (VDR).
The presence of chronic pain is associated with epigenetic modifications in genes responsible for the expression of enzymes involved in vitamin D metabolism and cellular function. These results lay groundwork in understanding the mechanism underlying the association between vitamin D and chronic pain.
最近的证据表明，维生素 D 可能与表观基因组相互作用，并在疼痛体验中发挥作用。为了使正常功能得以发挥，必须存在足够水平的维生素 D，这是通过允许维生素 D 具有生物活性的酶反应来实现的。本研究的目的是探讨维生素 D 代谢相关基因在有和没有慢性膝关节疼痛的个体中的表观遗传景观。
作为一项专注于膝关节疼痛的更大研究的一部分，社区居民提供了人口统计学、临床和与疼痛相关的信息，以及静脉血样，以确定 CpG 位点的 DNA 甲基化水平。
在编码与维生素 D 代谢相关的酶的基因中，有疼痛和无疼痛的个体之间的 DNA 甲基化存在差异：CYP27B1（1-α-羟化酶）。编码维生素 D 受体（VDR）的基因也存在过度甲基化。
慢性疼痛的存在与负责表达维生素 D 代谢和细胞功能相关酶的基因的表观遗传修饰有关。这些结果为理解维生素 D 与慢性疼痛之间关联的机制奠定了基础。
