Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, College of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China.
Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, College of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China.
Genomics. 2023 Mar;115(2):110592. doi: 10.1016/j.ygeno.2023.110592. Epub 2023 Feb 26.
Tomato is a widely cultivated fruit and vegetable and is valued for its flavor, colour, and nutritional value. C-aldehydes, such as (E)-2-hexenal, not only have antibacterial and antifungal properties but also function as signaling molecules that control the defense mechanisms of plants, including tomatoes. In this study, we used liquid chromatography-mass spectrometry (LC-MS) and RNA sequencing techniques to generate metabolome and transcriptome datasets that elucidate the molecular mechanisms regulating defense responses in tomato leaves exposed to (E)-2-hexenal. A total of 28.27 Gb of clean data were sequenced and assembled into 23,720 unigenes. In addition, a non-targeted metabolomics approach detected 739 metabolites. There were 233 significant differentially expressed genes (DEGs) (158 up-regulated, 75 down-regulated) and 154 differentially expressed metabolites (DEMs) (86 up-regulated, 69 down-regulated). Most nucleotides and amino acids (L-Phenylalanine, L-Asparagine, L-Histidine, L-Arginine, and L-Tyrosine) and their derivatives were enriched. The analyses revealed that mitogen-activated protein kinase (MPK), pathogenesis-related protein (PR), and endochitinase (CHIB) were primarily responsible for the adaptation of plant defense responses. Therefore, the extensive upregulation of these genes may be associated with the increased plant defense response. These findings help us comprehend the defense response of plants to (E)-2-hexenal and improve the resistance of horticultural plants.
番茄是一种广泛种植的水果和蔬菜，因其风味、颜色和营养价值而备受重视。C-醛类，如（E）-2-己烯醛，不仅具有抗菌和抗真菌特性，而且还作为信号分子，控制植物（包括番茄）的防御机制。在这项研究中，我们使用液相色谱-质谱（LC-MS）和 RNA 测序技术生成代谢组和转录组数据集，阐明了暴露于（E）-2-己烯醛的番茄叶片中调节防御反应的分子机制。共测序和组装了 28.27 Gb 的清洁数据，得到 23720 个非编码基因。此外，还采用非靶向代谢组学方法检测到 739 种代谢物。有 233 个显著差异表达基因（DEGs）（158 个上调，75 个下调）和 154 个差异表达代谢物（DEMs）（86 个上调，69 个下调）。大多数核苷酸和氨基酸（L-苯丙氨酸、L-天冬酰胺、L-组氨酸、L-精氨酸和 L-酪氨酸）及其衍生物都有富集。分析表明，丝裂原活化蛋白激酶（MPK）、病程相关蛋白（PR）和内切几丁质酶（CHIB）主要负责植物防御反应的适应。因此，这些基因的广泛上调可能与植物防御反应的增强有关。这些发现有助于我们理解植物对（E）-2-己烯醛的防御反应，并提高园艺植物的抗性。
