Liu Yuyao, Deng Yuan, Liao Dongyun, Han Yujie, Wang Qiang, Guo Yujing, Bai Hongcun, Dong Chuan, Fan Lifang
Institute of Environmental Science, College of Chemistry and Chemical Engineering, Shanxi University, Shanxi, 030006, Taiyuan, People's Republic of China.
State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, 030001, Shanxi, China.
Mikrochim Acta. 2023 Feb 28;190(3):107. doi: 10.1007/s00604-023-05670-w.
A label-free and efficient electrochemical (EC) sensing platform for di-2-ethylhexyl phthalate (DEHP) was developed based on in situ probe nickel hexacyanoferrate nanoparticle (NiHCF NP)-decorated three-dimensional reduced graphene oxide (3D rGO) composites. NiHCF NPs in the composites as an in situ probe show a pair of well-defined peaks with good reversibility and stability. Coupling 3D rGO with NiHCF NPs not only improved the electron transfer capability of NiHCF NPs but also provided more sites for aptamer immobilization. The synthesized NiHCF NP-decorated 3D rGO composites were used to act as a substrate for the immobilization of anti-DEHP aptamer by the covalent bonding method. The designed EC sensing platform displays excellent sensing performance for DEHP with a low detection limit of 3.64 pg/L, and a linear working range of 0.01 - 1000 ng/L. The application of the sensing platform to actual environmental samples was studied and satisfactory results were obtained. Thus, the proposed EC sensing platform would provide a potential tool for efficient detection of pollutants in the environment.
基于原位探针镍六氰合铁酸纳米颗粒（NiHCF NP）修饰的三维还原氧化石墨烯（3D rGO）复合材料，开发了一种用于邻苯二甲酸二（2-乙基己基）酯（DEHP）的无标记高效电化学（EC）传感平台。复合材料中的NiHCF NPs作为原位探针显示出一对定义明确的峰，具有良好的可逆性和稳定性。将3D rGO与NiHCF NPs耦合不仅提高了NiHCF NPs的电子转移能力，还为适配体固定提供了更多位点。合成的NiHCF NP修饰的3D rGO复合材料通过共价键合法用作固定抗DEHP适配体的底物。所设计的EC传感平台对DEHP表现出优异的传感性能，检测限低至3.64 pg/L，线性工作范围为0.01 - 1000 ng/L。研究了该传感平台在实际环境样品中的应用并获得了满意的结果。因此，所提出的EC传感平台将为环境中污染物的高效检测提供一种潜在工具。
