Wang Wenxue, Xu Hao, Zhao Baodong, Li Xin
Int J Periodontics Restorative Dent. 2023 Oct 24(7):s295-s306. doi: 10.11607/prd.6132.
Osteoporosis is an increasingly prevalent disease. The development of an osteoporosis-like experimental animal model is of great importance for the study of peri-implant osteogenesis in osteoporosis. The present authors aimed to establish a rapid modeling method of osteoporotic rabbits for implant-bone analysis and validate whether the models can affect the implant osseointegration. The present study included 29 female New Zealand rabbits (age: 5 to 6 months). Two rabbits were lost during anesthetization. Of the remaining 27 rabbits, 18 received an ovariectomy, with 9 receiving dexamethasone injections for 8 weeks (OVX+D group) and 9 receiving equivalent-volume saline injections (OVX group). As a control group, the remaining 9 rabbits were sham-operated and received an equivalent volume of normal saline (SHAM group). Then, serum biochemical markers of bone metabolism were detected and densitometric measurements were performed. Implants were then placed in the tibias of each rabbit. Bone samples (including implants) were obtained after 4, 8, and 12 weeks of healing and were subjected to histologic and histomorphometric analyses. The results showed that the OVX+D group experienced a 32% reduction in bone mineral density (BMD) from baseline. The BMD of the OVX+D group was significantly lower than that of the SHAM and OVX groups. Moreover, alkaline phosphatase (ALP) blood concentrations of in the OVX+D group were increased significantly. The osteoporotic rabbits exhibited marked decreases in osseointegration, characterized by slowed bone formation and decreased bone-to-implant contact (BIC). The combination of an ovariectomy and dexamethasone injections could experimentally induce osteoporosis in rabbits in the short term, which can be used as an appropriate animal model to study the osseointegration of implants under osteoporosis.
骨质疏松症是一种日益普遍的疾病。建立类似骨质疏松症的实验动物模型对于研究骨质疏松症患者种植体周围骨生成具有重要意义。本研究旨在建立一种用于种植体 - 骨分析的骨质疏松兔快速建模方法，并验证该模型是否会影响种植体骨整合。本研究纳入了29只雌性新西兰兔（年龄：5至6个月）。两只兔子在麻醉过程中死亡。在其余27只兔子中，18只接受了卵巢切除术，其中9只接受地塞米松注射8周（OVX + D组），9只接受等量生理盐水注射（OVX组）。作为对照组，其余9只兔子接受假手术并注射等量生理盐水（SHAM组）。然后，检测骨代谢的血清生化标志物并进行骨密度测量。随后将种植体植入每只兔子的胫骨中。在愈合4、8和12周后获取骨样本（包括种植体），并进行组织学和组织形态计量学分析。结果显示，OVX + D组的骨矿物质密度（BMD）较基线降低了32%。OVX + D组的BMD显著低于SHAM组和OVX组。此外，OVX + D组的碱性磷酸酶（ALP）血浓度显著升高。骨质疏松兔的骨整合明显降低，表现为骨形成减慢和骨与种植体接触（BIC）减少。卵巢切除术和地塞米松注射相结合可在短期内通过实验诱导兔骨质疏松，可作为研究骨质疏松状态下种植体骨整合的合适动物模型。
