[埃拉斯汀和G3139对慢性酒精中毒大鼠肝脏线粒体的影响]

[Effect of erastin and G3139 on rat liver mitochondria in chronic alcoholic intoxication].

作者信息

Baburina Yu L, Zvyagina A I, Odinokova I V, Krestinina O V

机构信息

Institute of Theoretical and Experimental Biophysics of the Russian Academy of Science, Pushchino, Russia.

出版信息

Biomed Khim. 2023 Feb;69(1):62-71. doi: 10.18097/PBMC20236901062.

DOI:10.18097/PBMC20236901062

PMID:36857428

Abstract

The effect of modulators of VDAC channels - G3139 and erastin on the mitochondrial permeability transition pore (mPTP) functioning and changes in the content of proteins involved in regulation of mPTP (VDAC, CNPase, and TSPO) has been investigated in liver mitochondria of rats with chronic alcohol intoxication. It was shown that the mitochondria of rats treated with ethanol were more sensitive to mPTP induction. Moreover, ethanol induced changes in the expression of mPTP regulator proteins. G3139 and erastin were also able to influence the studied mitochondrial parameters, and they increased their effect in the liver mitochondria of rats treated with ethanol, as compared to the mitochondria of control rats. We hypothesize that the results of this study may help to elucidate the mechanisms of chronic action of ethanol on mitochondria and contribute to the development of new therapeutic strategies for treating the consequences of ethanol-related diseases.

摘要

研究了电压依赖性阴离子通道（VDAC）调节剂G3139和艾拉司丁对慢性酒精中毒大鼠肝脏线粒体中，线粒体通透性转换孔（mPTP）功能以及参与mPTP调节的蛋白质（VDAC、2',3'-环核苷酸3'-磷酸二酯酶（CNPase）和外周型苯二氮䓬受体（TSPO））含量变化的影响。结果表明，乙醇处理的大鼠线粒体对mPTP诱导更敏感。此外，乙醇诱导了mPTP调节蛋白表达的变化。与对照大鼠的线粒体相比，G3139和艾拉司丁也能够影响所研究的线粒体参数，并且它们在乙醇处理的大鼠肝脏线粒体中的作用增强。我们推测，本研究结果可能有助于阐明乙醇对线粒体的慢性作用机制，并有助于开发治疗乙醇相关疾病后果的新治疗策略。