Demonceaux Marie, Goux Marine, Hendrickx Johann, Solleux Claude, Cadet Frédéric, Lormeau Émilie, Offmann Bernard, André-Miral Corinne
US2B, CNRS UMR 6286, Nantes University, Nantes 44300, France.
Laboratory of Excellence LABEX GR, DSIMB, Inserm UMR S1134, University of Paris City and University of Reunion, Paris 75014, France.
Org Biomol Chem. 2023 Mar 15;21(11):2307-2311. doi: 10.1039/d3ob00191a.
Mutation Q345F in sucrose phosphorylase from (SP) has shown to allow efficient (+)-catechin glucosylation yielding a regioisomeric mixture: (+)-catechin-3'--α-D-glucopyranoside, (+)-catechin-5--α-D-glucopyranoside and (+)-catechin-3',5--α-D-diglucopyranoside with a ratio of 51 : 25 : 24. Here, we efficiently increased the control of (+)-catechin glucosylation regioselectivity with a new variant Q345F/P134D. The same products were obtained with a ratio of 82 : 9 : 9. Thanks to bioinformatics models, we successfully explained the glucosylation favoured at the OH-3' position due to the mutation P134D.
来自[具体来源未提及]的蔗糖磷酸化酶（SP）中的Q345F突变已显示出能实现高效的（+）-儿茶素糖基化，产生一种区域异构体混合物：（+）-儿茶素-3'-α-D-吡喃葡萄糖苷、（+）-儿茶素-5-α-D-吡喃葡萄糖苷和（+）-儿茶素-3'，5-α-D-二吡喃葡萄糖苷，比例为51∶25∶24。在此，我们通过新变体Q345F/P134D有效地提高了对（+）-儿茶素糖基化区域选择性的控制。得到了相同的产物，比例为82∶9∶9。借助生物信息学模型，我们成功解释了由于P134D突变，OH-3'位置的糖基化更受青睐的原因。
