Singh Tejveer, Aggarwal Nikita, Thakur Kulbhushan, Chhokar Arun, Yadav Joni, Tripathi Tanya, Jadli Mohit, Bhat Anjali, Kumar Arun, Narula Ritika Hasija, Gupta Pankaj, Khurana Anil, Bharti Alok Chandra
Molecular Oncology Laboratory, Department of Zoology, University of Delhi, Delhi, India.
Department of Pharmacology, Dr. D.P. Rastogi Central Research Institute of Homeopathy, Noida, Uttar Pradesh, India.
Homeopathy. 2023 Nov;112(4):262-274. doi: 10.1055/s-0042-1756436. Epub 2023 Mar 1.
Plant-derived homeopathic medicines (HMs) are cheap and commercially available but are mechanistically less explored entities than conventional medicines.
The aim of our study was to evaluate the impact of selected plant-derived HMs derived from (BA) (BV), (MP), (CL), (CO), (TO) and (HC) on cervical cancer (CaCx) cells .
We screened the mother tincture (MT) and 30C potencies of the above-mentioned HMs for anti-proliferative and cytotoxic activity on human papillomavirus (HPV)-negative (C33a) and HPV-positive CaCx cells (SiHa and HeLa) by MTT assay. Total phenolic content (TPC) and the free-radical scavenging activity of each HM was also determined using standard assays. Phytochemicals reportedly available in these HMs were examined for their potential inhibitory action on HPV16 E6 by molecular docking.
All tested MTs induced a differential dose-dependent cytotoxic response that varied with cell line. For C33a cells, the order of response was TO > CL > BA > BV > HC > MP > CO, whereas for SiHa and HeLa cells the order was HC > MP > TO > CO > BA > BV > CL and CL > BA > CO, respectively. 30C potencies of all HMs showed an inconsistent response. Further, anti-CaCx responses displayed by MTs did not follow the order of an HM's phenolic content or free radical scavenging activity. Analysis revealed anti-oxidant content of BA, BV and HC had the lowest contribution to their anti-CaCx activity. Using modeling of molecular docking between the HPV16 E6 protein crystallographic structures (6SJA and 4XR8) and main phytochemical components of BV, BA, HC, CL and TO, their potential to inhibit the HPV16 E6 protein carcinogenic interactions was identified.
The study has shown a comparative evaluation of the potential of several plant-derived MTs and HMs to affect CaCx cell line survival (through cytotoxicity and free radical scavenging) and their theoretical molecular targets for the first time. Data demonstrated that MTs of BA and BV are likely to be the most potent HMs that strongly inhibited CaCx growth and have a strong anti-HPV phytochemical constitution.
植物源顺势疗法药物（HMs）价格低廉且可在市场上买到，但与传统药物相比，其作用机制的研究较少。
我们研究的目的是评估从紫花苜蓿（BA）、紫锥菊（BV）、报春花（MP）、土木香（CL）、薄荷（CO）、百里香（TO）和土木香（HC）中提取的选定植物源HMs对子宫颈癌（CaCx）细胞的影响。
我们通过MTT法筛选了上述HMs的母酊剂（MT）和30C药力对人乳头瘤病毒（HPV）阴性（C33a）和HPV阳性CaCx细胞（SiHa和HeLa）的抗增殖和细胞毒性活性。还使用标准测定法测定了每种HM的总酚含量（TPC）和自由基清除活性。通过分子对接研究了据报道这些HMs中含有的植物化学物质对HPV16 E6的潜在抑制作用。
所有测试的MT均诱导了不同的剂量依赖性细胞毒性反应，该反应因细胞系而异。对于C33a细胞，反应顺序为TO＞CL＞BA＞BV＞HC＞MP＞CO，而对于SiHa和HeLa细胞，反应顺序分别为HC＞MP＞TO＞CO＞BA＞BV＞CL和CL＞BA＞CO。所有HMs的30C药力均显示出不一致的反应。此外，MT显示的抗CaCx反应并不遵循HM酚含量或自由基清除活性的顺序。分析表明，BA、BV和HC的抗氧化剂含量对其抗CaCx活性的贡献最低。通过对HPV16 E6蛋白晶体结构（6SJA和4XR8）与BV、BA、HC、CL和TO的主要植物化学成分之间进行分子对接建模，确定了它们抑制HPV16 E6蛋白致癌相互作用的潜力。
该研究首次对几种植物源MT和HMs影响CaCx细胞系存活（通过细胞毒性和自由基清除）的潜力及其理论分子靶点进行了比较评估。数据表明，BA和BV的MT可能是最有效的HMs，它们强烈抑制CaCx生长并具有强大的抗HPV植物化学组成。
