知母杂多糖的结构特征及其抗氧化和降血糖活性
Structural characteristics and antioxidant and hypoglycemic activities of a heteropolysaccharide from Anemarrhena asphodeloides Bunge.
作者信息
Chen Juncheng, Li Lin, Zhang Xia, Zhang Yuan, Zheng Qingsong, Lan Meijuan, Li Bing
机构信息
School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China; International School of Public Health and One Health, Hainan Medical University, Haikou, Hainan 571199, China.
School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510640, China; School of Chemical Engineering and Energy Technology, Dongguan University of Technology, Dongguan 523808, China.
出版信息
Int J Biol Macromol. 2023 May 1;236:123843. doi: 10.1016/j.ijbiomac.2023.123843. Epub 2023 Feb 27.
In this study, an acid polysaccharide (AABP-1B) was extracted from the rhizome of Anemarrhena asphodeloides Bunge and purified using 60 % alcohol precipitation and DEAE-52 cellulose. The molecular weight of AABP-1B was 105 kDa, and it consisted of mannose (Man), rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara) in a ratio of 6.3:1.3:1.1:0.2:0.4:0.7. Methylation and NMR analyses revealed that the backbone of AABP-1 consists of 4)-β-D-Manp-(1 and 4)-2-O-acetyl-β-D-Manp-(1. In addition, the biological activity assays showed that AABP-1B not only displays potential antioxidant activity but also exhibits the α-glucosidase and α-amylase inhibitory effect. Moreover, AABP-1B enhanced glucose consumption and glycogen synthesis in insulin-resistant (IR) HepG2 cells. These results suggest that AABP-1B has potential hypoglycemic activity.
在本研究中，从知母根茎中提取了一种酸性多糖（AABP-1B），并使用60%乙醇沉淀和DEAE-52纤维素进行纯化。AABP-1B的分子量为105 kDa，它由甘露糖（Man）、鼠李糖（Rha）、半乳糖醛酸（GalA）、葡萄糖（Glc）、半乳糖（Gal）和阿拉伯糖（Ara）以6.3:1.3:1.1:0.2:0.4:0.7的比例组成。甲基化和核磁共振分析表明，AABP-1的主链由4)-β-D-甘露糖基-(1和4)-2-O-乙酰基-β-D-甘露糖基-(1组成。此外，生物活性测定表明，AABP-1B不仅具有潜在的抗氧化活性，还表现出α-葡萄糖苷酶和α-淀粉酶抑制作用。此外，AABP-1B增强了胰岛素抵抗（IR）HepG2细胞中的葡萄糖消耗和糖原合成。这些结果表明，AABP-1B具有潜在的降血糖活性。
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