Yang Hui-Rong, Chen Lian-Hong, Zeng Ying-Jie
College of Food Science & Technology, Southwest Minzu University, Chengdu 610041, China.
Foods. 2021 Sep 14;10(9):2184. doi: 10.3390/foods10092184.
The structure, antioxidant activity and hypoglycemic activity in vitro of a novel homogeneous polysaccharide from (Tmp) were investigated. Structural features suggested that Tmp was consisted of arabinose (Ara), mannose (Man), glucose (Glc) and galactose (Gal) with a molar ratio of 1.9:13.6:42.7:28.3, respectively, with a molecular weight of 72.14 kDa. The structural chain of Tmp was confirmed to contain →2,5)-α-l-Arabinofuranose (Ara)-(1→, →3,5)-α-l-Ara-(1→, β-d-Glucopyranose (Glc)-(1→, α-d-Mannopyranose (Man)-(1→, α-d-Galacopyranose (Gal)-(1→, →4)-β-d-Gal-(1→, →3)-β-d-Glc-(1→, →3)-α-d-Man-(1→, →6)-3--Methyl (Me)-α-d-Man-(1→, →6)-α-d-Gal-(1→, →3,6)-β-d-Glc-(1→, →6)-α-d-Man-(1→ residues. Furthermore, Tmp possessed strong antioxidant activity and showed the strong inhibitory effect on α-glucosidase and α-amylase activities. Then, a further evaluation found that there was a dramatic improvement in the glucose consumption, glycogen synthesis and the activities of pyruvate kinase and hexokinase when the insulin-resistant-human hepatoma cell line (IR-HepG2) was treated with Tmp. The above results indicated that Tmp had good hypoglycemic activity and also exhibited great potentials in in terms of dealing with type 2 diabetes mellitus.
对从[来源未提及]中提取的一种新型均一多糖（Tmp）的结构、抗氧化活性及体外降血糖活性进行了研究。结构特征表明，Tmp由阿拉伯糖（Ara）、甘露糖（Man）、葡萄糖（Glc）和半乳糖（Gal）组成，其摩尔比分别为1.9:13.6:42.7:28.3，分子量为72.14 kDa。Tmp的结构链经确认含有→2,5)-α-L-阿拉伯呋喃糖（Ara）-(1→、→3,5)-α-L-Ara-(1→、β-D-葡萄糖吡喃糖（Glc）-(1→、α-D-甘露糖吡喃糖（Man）-(1→、α-D-半乳糖吡喃糖（Gal）-(1→、→4)-β-D-Gal-(1→、→3)-β-D-Glc-(1→、→3)-α-D-Man-(1→、→6)-3--甲基（Me）-α-D-Man-(1→、→6)-α-D-Gal-(1→、→3,6)-β-D-Glc-(1→、→6)-α-D-Man-(1→残基。此外，Tmp具有较强的抗氧化活性，并对α-葡萄糖苷酶和α-淀粉酶活性表现出较强的抑制作用。进一步评估发现，用Tmp处理胰岛素抵抗人肝癌细胞系（IR-HepG2）时，葡萄糖消耗、糖原合成以及丙酮酸激酶和己糖激酶的活性有显著改善。上述结果表明，Tmp具有良好的降血糖活性，在治疗2型糖尿病方面也具有巨大潜力。
