Nevinsky G A, Lavrik O I, Favorova O O, Kisselev L L
Mol Biol Rep. 1978 Oct 16;4(3):181-4. doi: 10.1007/BF00777521.
A simple and rapid column procedure is described for the isolation from protein hydrolysates of peptides containing covalently bound substrate analogues with cis-diol groups. The method is based on complex formation between the cis-diol groups of peptide-bound compounds and dihydroxyborylic groups of a dihydroxyborylaminoethyl cellulose column. The method is useful for isolation of peptide(s) located in or near the active centre of enzymes after their affinity labelling by chemically active analogues of natural substrates like ribonucleotides, sugars, etc.
