Wuri Nile, Gou Hongchao, Zhang Bin, Wang Menglu, Wang Songqi, Zhang Weixiao, He Haiyan, Fan Xuelei, Zhang Chunhong, Liu Zhicheng, Geri Letu, Shen Haiyan, Zhang Jianfeng
Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Front Vet Sci. 2023 Feb 13;10:1116695. doi: 10.3389/fvets.2023.1116695. eCollection 2023.
Porcine epidemic diarrhea virus (PEDV) is a deadly pathogen infecting pig herds, and has caused significant economic losses around the world. Vaccination remains the most effective way of keeping the PEDV epidemic under control. Previous studies have shown that the host metabolism has a significant impact on viral replication. In this study, we have demonstrated that two substrates of metabolic pathway, glucose and glutamine, play a key role in PEDV replication. Interestingly, the boosting effect of these compounds toward viral replication appeared to be dose-independent. Furthermore, we found that lactate, which is a downstream metabolite, promotes PEDV replication, even when added in excess to the cell culture medium. Moreover, the role of lactate in promoting PEDV was independent of the genotype of PEDV and the multiplicity of infection (MOI). Our findings suggest that lactate is a promising candidate for use as a cell culture additive for promoting PEDV replication. It could improve the efficiency of vaccine production and provide the basis for designing novel antiviral strategies.
猪流行性腹泻病毒（PEDV）是一种感染猪群的致命病原体，已在全球范围内造成重大经济损失。疫苗接种仍然是控制PEDV疫情的最有效方法。先前的研究表明，宿主代谢对病毒复制有重大影响。在本研究中，我们证明了代谢途径的两种底物，即葡萄糖和谷氨酰胺，在PEDV复制中起关键作用。有趣的是，这些化合物对病毒复制的促进作用似乎与剂量无关。此外，我们发现乳酸作为下游代谢产物，即使在细胞培养基中过量添加，也能促进PEDV复制。此外，乳酸促进PEDV的作用与PEDV的基因型和感染复数（MOI）无关。我们的研究结果表明，乳酸是用作促进PEDV复制的细胞培养添加剂的有前途的候选物。它可以提高疫苗生产效率，并为设计新型抗病毒策略提供依据。
