Yamamoto K I, Kikuchi Y
Mutat Res. 1978 Nov;52(2):207-9. doi: 10.1016/0027-5107(78)90142-2.
After the seminiferous tubules were minced and washed with phosphate-buffered saline to remove sperms, spermatids and spermatocytes (Hoo and Bowles, 1971), they were repeatedly treated with 0.1% trypsin solution to liberate spermatogonia. It was concluded that the spermatogonial chromosomes can be analysed much more easily and accurately by this procedure than by previous ones.