Laboratory of Food Chemistry and Nutrition, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Naval Architecture and Port Engineering College, Shandong Jiaotong University, Weihai, 264209, China.
Curr Microbiol. 2023 Mar 2;80(4):121. doi: 10.1007/s00284-023-03216-0.
Arsenic (As) exists widely in the environment and its strong toxicity endangers human health, causing widespread concern. Microbial adsorption technology plays an important role in As removal due to its advantages of high safety, low pollution, and low cost. The removal of As by active microorganisms requires not only good accumulation characteristics but also high As tolerance. The effect of salt preincubation on arsenate [As(V)] tolerance and bioaccumulation of Pichia kudriavzevii A16 and the possible mechanisms were studied. Salt preincubation improved the As(V) tolerance and bioaccumulation ability of the yeast. After NaPO preincubation, the proportion of dead cells and cells with high reactive oxygen species (ROS) accumulation decreased from 50.88% and 16.54% to 14.60% and 5.24%, respectively. In addition, the As removal rate significantly increased from 26.20% to 57.98%. The preincubated cells showed stronger As(V) tolerance and removal ability. The potential of use in complex environment to remove As(V) as well as the mechanisms involved in As(V) tolerance by yeast will be discussed.
砷（As）广泛存在于环境中，其强毒性危害人类健康，引起广泛关注。微生物吸附技术因其安全性高、污染低、成本低等优点，在砷去除中发挥着重要作用。活性微生物对砷的去除不仅需要良好的积累特性，还需要高砷耐受性。本研究探讨了盐预培养对毕赤酵母 A16 耐砷酸盐 [As(V)] 和生物积累的影响及其可能的机制。盐预培养提高了酵母的 As(V)耐受性和生物积累能力。经 NaPO 预培养后，死细胞和高活性氧（ROS）积累细胞的比例分别从 50.88%和 16.54%下降至 14.60%和 5.24%。此外，砷去除率从 26.20%显著提高至 57.98%。预培养细胞表现出更强的 As(V)耐受性和去除能力。将讨论酵母对 As(V)的耐受性及其在复杂环境中去除 As(V)的应用潜力。
