Patole Vinita Chandrakant, Awari Devyani, Chaudhari Shilpa
Dr. D.Y. Patil Institue of Pharmaceutical Sciences and Research, Department of Pharmaceutics, Pune, India.
Datta Meghe Institute of Medical Sciences (Deemed to be University), Datta Meghe College of Pharmacy, Maharashtra, India.
Turk J Pharm Sci. 2023 Mar 2;20(1):23-34. doi: 10.4274/tjps.galenos.2022.93275.
The study was aimed to formulate resveratrol (RSV) loaded microsponges to deliver drug at the wound site and incorporate it in the Lam. (Moringaceae) gel base to provide an appropriate moist environment for wound management. RSV, a stilbenoid that activates sirtuins and cell-signaling regulators involved in the process of wound healing.
Microsponges were prepared by oil in oil emulsion solvent diffusion method by optimizing the independent variables; drug: polymer ratio and volume of internal phase solvent and their effects on entrapment efficiency and particle size. Formulation batches were evaluated for drug content, production yield, entrapment efficiency, and drug release. The microsponges were further incorporated into gum gel, which was then evaluated for spreadability, viscosity, diffusion study and studies using an excision wound model in rats.
Scanning electron microscopy revealed spherical and porous nature of the microsponges -release study of the optimized batch of RSV microsponges showed 80.88% drug release within 8 h. Differential scanning calorimetry results revealed no drug and polymer interaction during the formation of microsponges. An diffusion study through goat skin revealed sustained release of RSV through porous microsponges embedded in the gel base at the wound site. An study performed using an excision wound model showed wound healing and closure within day 8. Histopathology showed increased re-epithelization and reduced ulceration in RSV microsponge gel-treated group compared with sham operated.
RSV microsponge gel delivered the drug at the wound site and the gel base provided a moist environment and influenced cell adhesion, thereby promoting faster wound healing.
本研究旨在制备负载白藜芦醇（RSV）的微海绵，以便在伤口部位给药，并将其纳入辣木科植物辣木凝胶基质中，为伤口处理提供适宜的湿润环境。RSV是一种芪类化合物，可激活参与伤口愈合过程的沉默调节蛋白和细胞信号调节因子。
采用油包油乳液溶剂扩散法制备微海绵，通过优化自变量（药物与聚合物比例、内相溶剂体积）及其对包封率和粒径的影响。对制剂批次进行药物含量、产率、包封率和药物释放的评估。将微海绵进一步掺入辣木凝胶中，然后对其进行铺展性、粘度、扩散研究以及在大鼠切除伤口模型上的研究。
扫描电子显微镜显示微海绵呈球形且具有多孔性。优化批次的RSV微海绵的释放研究表明，8小时内药物释放率为80.88%。差示扫描量热法结果显示，在微海绵形成过程中药物与聚合物无相互作用。通过山羊皮肤的扩散研究表明，RSV通过嵌入伤口部位凝胶基质中的多孔微海绵持续释放。使用切除伤口模型进行的研究显示，在第8天伤口愈合并闭合。组织病理学显示，与假手术组相比，RSV微海绵凝胶治疗组的上皮再形成增加，溃疡减少。
RSV微海绵凝胶在伤口部位给药，凝胶基质提供了湿润环境并影响细胞粘附，从而促进伤口更快愈合。
