Zhang Mingzhi, Sun Yang
Institute of Molecular Medicine (IMM), Renji Hospital, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Chem. 2023 Feb 14;11:1140022. doi: 10.3389/fchem.2023.1140022. eCollection 2023.
Information on the temporal and spatial scale of cellular molecules in biological systems is crucial for estimating life processes and may be conducive to an improved understanding of disease progression. This intracellular and extracellular information is often difficult to obtain at the same time due to the limitations of accessibility and sensing throughput. DNA is an excellent material for and applications, and can be used to build functional modules that can transform bio-information (input) into ATCG sequence information (output). Due to their small volume and highly amenable programming, DNA-based functional modules provide an opportunity to monitor a range of information, from transient molecular events to dynamic biological processes. Over the past two decades, with the advent of customized strategies, a series of functional modules based on DNA networks have been designed to gather different information about molecules, including the identity, concentration, order, duration, location, and potential interactions; the action of these modules are based on the principle of kinetics or thermodynamics. This paper summarizes the available DNA-based functional modules that can be used for biomolecular signal sensing and transformation, reviews the available designs and applications of these modules, and assesses current challenges and prospects.
生物系统中细胞分子的时空尺度信息对于评估生命过程至关重要，可能有助于更好地理解疾病进展。由于可及性和传感通量的限制，这种细胞内和细胞外信息通常难以同时获取。DNA是用于[此处原文缺失部分内容]和[此处原文缺失部分内容]应用的优良材料，可用于构建能够将生物信息（输入）转化为ATCG序列信息（输出）的功能模块。基于DNA的功能模块体积小且易于编程，提供了一个监测从瞬时分子事件到动态生物过程等一系列信息的机会。在过去二十年中，随着定制策略的出现，一系列基于DNA网络的功能模块被设计用于收集有关分子的不同信息，包括身份、浓度、顺序、持续时间、位置和潜在相互作用；这些模块的作用基于动力学或热力学原理。本文总结了可用于生物分子信号传感和转化的基于DNA的功能模块，回顾了这些模块的现有设计和应用，并评估了当前的挑战和前景。
