Zhao Jinchai, Chen Wei, Liu Jian
Department of Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, 050000, China.
Department of Pharmacy, The Second Hospital of Hebei Medical University, Shijiazhuang City, Hebei Province, 050000, China.
Open Med (Wars). 2023 Feb 25;18(1):20230638. doi: 10.1515/med-2023-0638. eCollection 2023.
Decreased locomotor activity and altered urinary frequency are induced by bilateral common iliac vein ligation in rats. As a carotenoid, lycopene has a strong anti-oxidative function. This research investigated the function of lycopene in the pelvic venous congestion (PC) rat model and the underlying molecular mechanism. Lycopene and olive oil were administered intragastrically on a daily basis for 4 weeks after successful modeling. Locomotor activity, voiding behavior, and continuous cystometry were analyzed. The levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), nitrate and nitrite (NO ), and creatinine in the urine were measured. Gene expression in the bladder wall was analyzed by quantitative reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blot. Locomotor activity, single voided volume, the interval between the bladder contractions, and urinary NO /cre ratio were all decreased in rats with PC, while the frequency of urination, urinary 8-OHdG/cre ratio, inflammatory responses, and nuclear factor-κB (NF-κB) signal activity were all increased. Lycopene treatment increased locomotor activity, decreased frequency of urination, elevated urinary NO level, and decreased urinary 8-OHdG level in the PC rat model. Lycopene also inhibited PC-enhanced pro-inflammatory mediator expression and NF-κB signaling pathway activity. In conclusion, lycopene treatment ameliorates PC-induced phenotypes and shows an anti-inflammatory effect in the PC rat model.
双侧髂总静脉结扎可导致大鼠运动活性降低和排尿频率改变。作为一种类胡萝卜素，番茄红素具有强大的抗氧化功能。本研究探讨了番茄红素在盆腔静脉淤血（PC）大鼠模型中的作用及其潜在分子机制。成功建模后，每天经胃给予番茄红素和橄榄油，持续4周。分析运动活性、排尿行为和连续膀胱测压。检测尿液中8-羟基-2'-脱氧鸟苷（8-OHdG）、硝酸盐和亚硝酸盐（NO ）以及肌酐的水平。通过定量逆转录聚合酶链反应、酶联免疫吸附测定和蛋白质印迹法分析膀胱壁中的基因表达。PC大鼠的运动活性、单次排尿量、膀胱收缩间隔时间和尿液NO /肌酐比值均降低，而排尿频率、尿液8-OHdG/肌酐比值、炎症反应和核因子κB（NF-κB）信号活性均增加。番茄红素治疗可增加PC大鼠模型的运动活性，降低排尿频率，提高尿液NO 水平，降低尿液8-OHdG水平。番茄红素还可抑制PC增强的促炎介质表达和NF-κB信号通路活性。总之，番茄红素治疗可改善PC诱导的表型，并在PC大鼠模型中显示出抗炎作用。
