Lebedeva N N, Wrotek M, Shuvalova L A, Konkol I, Borovikov Iu S
Tsitologiia. 1987 Aug;29(8):973-5.
A simple method for obtaining glycerinated muscle fibres of m. psoas of rabbit containing regulatory myosin light chains (LC2) of different levels of phosphorylation. The glycerination conditions stimulated endogenic kinase LC2 or phosphatase LC2. Glycerinated muscle fibres contained phosphorylated and dephosphorylated (levels of phosphorylation are 95 +/- 5%, and 5 +/- 5%, respectively) LC2 of myosin. To determine the level of phosphorylation the method of polyacrylamide gel electrophoresis in 8 M urea was modified.
一种获取兔腰大肌甘油化肌纤维的简单方法，该肌纤维含有不同磷酸化水平的调节性肌球蛋白轻链（LC2）。甘油化条件激活了内源性激酶LC2或磷酸酶LC2。甘油化肌纤维含有磷酸化和去磷酸化的（磷酸化水平分别为95±5%和5±5%）肌球蛋白LC2。为了测定磷酸化水平，对8M尿素中的聚丙烯酰胺凝胶电泳方法进行了改进。
