Sukhonthasilakun Sumrit, Mahakunakorn Pramote, Naladta Alisa, Nuankaew Katesaraporn, Nualkaew Somsak, Yenjai Chavi, Nualkaew Natsajee
Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Biochemistry, Faculty of Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
J Ayurveda Integr Med. 2023 Mar-Apr;14(2):100693. doi: 10.1016/j.jaim.2023.100693. Epub 2023 Mar 1.
Narrowband-ultraviolet B (NB-UVB) has been used to treat skin diseases such as psoriasis. Chronic use of NB-UVB might cause skin inflammation and lead to skin cancer. In Thailand, Derris Scandens (Roxb.) Benth. is used as an alternative medicine to nonsteroidal anti-inflammatory drugs (NSAIDs) for low back pain and osteoarthritis. Therefore, this study aimed to evaluate the potential anti-inflammatory effect of Derris scandens extract (DSE) on pre- and post exposed NB-UVB human keratinocytes (HaCaT). The results indicated that DSE could not protect HaCaT from cell morphology changes or DNA fragmentation and could not recover cell proliferation ability from the NB-UVB effects. DSE treatment reduced the expression of genes related to inflammation, collagen degradation, and carcinogenesis, such as IL-1α, IL-1β, IL-6, iNOS, COX-2, MMP-1, MMP-9, and Bax. These results indicated the potential use of DSE as a topical preparation against NB-UVB-induced inflammation, anti-aging, and prevention of skin cancer from phototherapy.
窄谱中波紫外线（NB - UVB）已被用于治疗银屑病等皮肤病。长期使用NB - UVB可能会引起皮肤炎症并导致皮肤癌。在泰国，鱼藤被用作非甾体抗炎药（NSAIDs）的替代药物，用于治疗腰痛和骨关节炎。因此，本研究旨在评估鱼藤提取物（DSE）对暴露于NB - UVB前后的人角质形成细胞（HaCaT）的潜在抗炎作用。结果表明，DSE不能保护HaCaT细胞免受细胞形态变化或DNA片段化的影响，也不能从NB - UVB的影响中恢复细胞增殖能力。DSE处理降低了与炎症、胶原蛋白降解和致癌作用相关的基因表达，如IL - 1α、IL - 1β、IL - 6、诱导型一氧化氮合酶（iNOS）、环氧化酶 - 2（COX - 2）、基质金属蛋白酶 - 1（MMP - 1）、基质金属蛋白酶 - 9（MMP - 9）和凋亡蛋白Bax。这些结果表明DSE作为一种局部制剂在对抗NB - UVB诱导的炎症、抗老化以及预防光疗引起的皮肤癌方面具有潜在用途。
