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马鞭草科植物皇后花环(Petrea volubilis L.)的染色体水平组装。

Chromosome-scale assembly of the Verbenaceae species Queen's Wreath (Petrea volubilis L.).

机构信息

Center for Applied Genetic Technologies, University of Georgia, Athens, GA, 30602, USA.

Department of Crop & Soil Sciences, University of Georgia, Athens, GA, 30602, USA.

出版信息

BMC Genom Data. 2023 Mar 3;24(1):14. doi: 10.1186/s12863-023-01110-z.

Abstract

OBJECTIVES

Petrea volubilis, a member of the Order Lamiales and the Verbenaceae family, is an important horticultural species that has been used in traditional folk medicine. To provide a genome sequence for comparative studies within the Order Lamiales that includes important families such as Lamiaceae (mints), we generated a long-read, chromosome-scale genome assembly of this species.

DATA DESCRIPTION

Using a total of 45.5 Gb of Pacific Biosciences long read sequence, we generated a 480.2 Mb assembly of P. volubilis, of which, 93% is chromosome anchored. Representation of genic regions was robust with 96.6% of the Benchmarking of Universal Single Copy Orthologs present in the genome assembly. A total of 57.8% of the genome was annotated as a repetitive sequence. Using a gene annotation pipeline that included refinement of gene models using transcript evidence, 30,982 high confidence genes were annotated. Access to the P. volubilis genome will facilitate evolutionary studies in the Lamiales, a key order of Asterids that includes significant crop and medicinal plant species.

摘要

目的

Petrea volubilis 是玄参目马鞭草科的一种重要园艺物种,它是唇形目(薄荷科)和马鞭草科(马鞭草科)等重要科的传统民间医学中的重要成员。为了在包括唇形目(薄荷科)和马鞭草科(马鞭草科)在内的 Lamiales 目中进行比较研究提供基因组序列,我们生成了该物种的长读长、染色体尺度基因组组装。

数据描述

使用总共 45.5GB 的太平洋生物科学长读序列,我们生成了 480.2MB 的 P.volubilis 组装,其中 93%是染色体锚定的。基因区域的代表性很强,基因组组装中存在 96.6%的基准通用单拷贝直系同源基因。基因组的总共有 57.8%被注释为重复序列。使用包括使用转录证据细化基因模型的基因注释管道,注释了 30982 个高可信度基因。访问 P.volubilis 基因组将促进 Lamiales 中的进化研究,Lamiales 是一个关键的 Asterids 目,其中包括重要的作物和药用植物物种。

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