Department of Symptom Research, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Behav Brain Res. 2023 Apr 27;444:114381. doi: 10.1016/j.bbr.2023.114381. Epub 2023 Mar 3.
Based on previous results showing a pivotal role of endogenous interleukin-10 (IL-10) in the recovery from cisplatin-induced peripheral neuropathy, the present experiments were carried out to determine whether this cytokine plays any role in the recovery from cisplatin-induced fatigue in male mice. Fatigue was measured by decreased voluntary wheel running in mice trained to run in a wheel in response to cisplatin. Mice were treated with a monoclonal neutralizing antibody (IL-10na) administered intranasally during the recovery period to neutralize endogenous IL-10. In the first experiment, mice were treated with cisplatin (2.83 mg/kg/day) for five days and IL-10na (12 μg/day for three days) five days later. In the second experiment, they were treated with cisplatin (2.3 mg/kg/day for 5 days twice at a five-day interval) and IL10na (12 μg/day for three days) immediately after the last injection of cisplatin. In both experiments, cisplatin decreased body weight and reduced voluntary wheel running. However, IL-10na did not impair recovery from these effects. These results show that the recovery from the cisplatin-induced decrease in wheel running does not require endogenous IL-10 in contrast to the recovery from cisplatin-induced peripheral neuropathy.
基于先前的研究结果表明，内源性白细胞介素-10（IL-10）在顺铂诱导的周围神经病变的恢复中起着关键作用，本实验旨在确定这种细胞因子是否在顺铂诱导的雄性小鼠疲劳恢复中起作用。疲劳通过训练在轮子上跑步的小鼠在顺铂作用下自愿跑步减少来测量。在恢复期，用鼻内给予单克隆中和抗体（IL-10na）来中和内源性 IL-10 来治疗小鼠。在第一个实验中，用顺铂（2.83mg/kg/天）治疗 5 天，5 天后用 IL-10na（12μg/天，3 天）治疗。在第二个实验中，他们用顺铂（2.3mg/kg/天，5 天，间隔 5 天两次）和 IL10na（12μg/天，3 天）在最后一次顺铂注射后立即治疗。在两个实验中，顺铂均降低体重并减少自愿轮跑。然而，IL-10na 并没有损害从这些影响中恢复。这些结果表明，与顺铂诱导的周围神经病变的恢复不同，顺铂诱导的轮跑减少的恢复不需要内源性 IL-10。
