激光和光动力疗法对受龋齿影响牙本质（乳杆菌属）消毒后，对生物活性和大体积充填树脂的黏结。
Adhesive bond of Bioactive and bulk fill resin to disinfected caries affected dentin (lactobacillus specie) with laser and photodynamic therapy.
机构信息
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
出版信息
Photodiagnosis Photodyn Ther. 2023 Jun;42:103493. doi: 10.1016/j.pdpdt.2023.103493. Epub 2023 Mar 2.
AIMS
To evaluate the antimicrobial effectiveness of cavity disinfectants chlorhexidine gluconate (CHX), Er, Cr, YSGG laser (ECL), and curcumin photosensitizer (CP) against Lactobacillus and shear bond strength (SBS) of Bioactive (BA) and bulk fill composite (BFC) restorative material bonded to carious affected dentin (CAD).
MATERIALS AND METHODS
Sixty human mandibular molars scored 4 and 5 on the International Caries Detection and Assessment System (ICDAS) were included. After inoculating the specimens with lactobacillus specie all the samples were arbitrarily divided into three groups based on the disinfection regime used (n=20). Groups 1 and 2: CAD disinfection using ECL, Groups 3 and 4: CAD disinfection using CP, and Groups 5 and 6: CAD disinfection using CHX. After cavity sterilization, the survival rate was estimated and each group was further divided into two sub-groups based on the restorative material used. Groups 1, 3, and 5 (n = 10) were restored using BFC restorative material, and groups 2, 4, and 6 (n = 10) were restored using a conventional bulk-fill resin material. A universal testing machine (UTM) was used to determine the SBS and debonded surfaces were examined under a stereomicroscope to determine the modes of failure. Kruskal-Walis, ANOVA, and Post Hoc Tukey were applied to investigate the survival rate and bond strength values.
RESULTS
ECL group displayed the highest survival rate (0.73±0.13) of Lactobacillus. The least survival rate (0.17±0.09) was demonstrated by CP activated by PDT. Group 1 (ECL+ BA) treated specimens exhibited the maximum value of SBS (18.31 ± 0.22 MPa). However, group 3 (CP + BA) revealed the minimum values of bond strength (14.05 ± 1.02 MPa). The intergroup comparison revealed that group 1, group 2 (ECL+BFC) (18.11 ± 0.14 MPa), group 5 (CHX+ BA) (18.14 ± 0.36 MPa), and group 6 (CHX+BFC) (18.18 ± 0.35 MPa) displayed comparable outcomes of bond integrity (p>0.05).
CONCLUSION
Caries-affected dentin disinfected with Er, Cr: YSGG, and chlorhexidine improve bond scores of bioactive and conventional bulk-fill restorative material.
目的
评估腔消毒剂葡萄糖酸氯己定（CHX）、Er、Cr、YSGG 激光（ECL）和姜黄素光敏剂（CP）对乳酸杆菌的抗菌效果，以及生物活性（BA）和块状填充复合材料（BFC）修复材料与龋损牙本质（CAD）的粘结强度（SBS）。
材料和方法
纳入 60 个人类下颌磨牙，在国际龋病检测和评估系统（ICDAS）上评分为 4 和 5。在将标本接种乳酸杆菌后，所有样本均根据使用的消毒方案任意分为三组（n=20）。第 1 组和第 2 组：使用 ECL 对 CAD 进行消毒，第 3 组和第 4 组：使用 CP 对 CAD 进行消毒，第 5 组和第 6 组：使用 CHX 对 CAD 进行消毒。腔消毒后，估计存活率，然后根据使用的修复材料将每组进一步分为两个亚组。第 1、3 和 5 组（n=10）用 BFC 修复材料修复，第 2、4 和 6 组（n=10）用常规块状填充树脂材料修复。使用万能试验机（UTM）测定 SBS，并用立体显微镜检查离解表面，以确定失效模式。应用 Kruskal-Wallis、方差分析和事后 Tukey 检验来研究存活率和粘结强度值。
结果
ECL 组显示出最高的乳酸杆菌存活率（0.73±0.13）。PDT 激活的 CP 显示出最低的存活率（0.17±0.09）。第 1 组（ECL+BA）处理标本的 SBS 值最大（18.31 ± 0.22 MPa）。然而，第 3 组（CP+BA）显示出最小的粘结强度值（14.05 ± 1.02 MPa）。组间比较显示，第 1 组、第 2 组（ECL+BFC）（18.11 ± 0.14 MPa）、第 5 组（CHX+BA）（18.14 ± 0.36 MPa）和第 6 组（CHX+BFC）（18.18 ± 0.35 MPa）的粘结完整性结果相似（p>0.05）。
结论
用 Er、Cr：YSGG 和氯己定消毒龋损牙本质可提高生物活性和常规块状填充修复材料的粘结分数。
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