Oliyaei Najmeh, Tanideh Nader, Moosavi-Nasab Marzieh, Dehghanian Amir Reza, Iraji Aida
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Seafood Processing Research Center, School of Agriculture, Shiraz University, Shiraz, Iran; Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Biol Macromol. 2023 Apr 30;235:123867. doi: 10.1016/j.ijbiomac.2023.123867. Epub 2023 Mar 3.
The anti-obesity activity of encapsulated fucoxanthin in fucoidan-based nanoemulsion was investigated. Then, high-fat diet (HFD) induced-obese rats were fed along with different treatments including administration of encapsulated fucoxanthin (10 mg/kg and 50 mg/kg/day), fucoidan (70 mg/kg), Nigella sativa oil (250 mg/kg), metformin (200 mg/kg), and free form of fucoxanthin (50 mg/kg) by oral gavage daily for 7 weeks. The study discovered that fucoidan-based nanoemulsions with a low and high dose of fucoxanthin had droplet size in the range of 181.70-184.87 nm and encapsulation efficacy of 89.94-91.68 %, respectively. Also exhibited 75.86 % and 83.76 % fucoxanthin in vitro release. The TEM images and FTIR spectera confirmed the particle size and encapsulation of fucoxanthin, respectively. Moreover, in vivo results revealed that encapsulated fucoxanthin reduced body and liver weight compared with a HFD group (p < 0.05). Biochemical parameters (FBS, TG, TC, HDL, LDL) and liver enzymes (ALP, AST, and ALT) were decreased after fucoxanthin and fucoidan administration. According to the histopathological analysis, fucoxanthin and fucoidan attenuated lipid accumulation in the liver.
研究了岩藻依聚糖基纳米乳剂中包封的岩藻黄质的抗肥胖活性。然后，对高脂饮食（HFD）诱导的肥胖大鼠进行不同处理，包括每日经口灌胃给予包封的岩藻黄质（10毫克/千克和50毫克/千克/天）、岩藻依聚糖（70毫克/千克）、黑种草籽油（250毫克/千克）、二甲双胍（200毫克/千克）和游离形式的岩藻黄质（50毫克/千克），持续7周。研究发现，低剂量和高剂量岩藻黄质的岩藻依聚糖基纳米乳剂的液滴尺寸分别在181.70 - 184.87纳米范围内，包封率为89.94 - 91.68%。体外释放的岩藻黄质分别为75.86%和83.76%。透射电子显微镜（TEM）图像和傅里叶变换红外光谱（FTIR）分别证实了岩藻黄质的粒径和包封情况。此外，体内结果显示，与高脂饮食组相比，包封的岩藻黄质降低了体重和肝脏重量（p < 0.05）。给予岩藻黄质和岩藻依聚糖后，生化参数（空腹血糖、甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白）和肝酶（碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶）降低。根据组织病理学分析，岩藻黄质和岩藻依聚糖减轻了肝脏中的脂质积累。
