Sielski Galvão Soares Luana, Casella Tiago, Kawagoe Eric Kazuo, Benetti Filho Vilmar, Omori Wellington Pine, Nogueira Mara Corrêa Lelles, Wagner Glauber, Rodrigues de Oliveira Rafael, Stahlhofer Sabine Rippel, Antunes Ferreira Fabienne, Tondo Eduardo Cesar, De Dea Lindner Juliano
Food Technology and Bioprocess Research Group, Department of Food Science and Technology, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.
Center for Microorganisms Investigation, São José Do Rio Preto Medical School (FAMERP), São José Do Rio Preto, SP, Brazil.
Int J Food Microbiol. 2023 Apr 16;391-393:110151. doi: 10.1016/j.ijfoodmicro.2023.110151. Epub 2023 Feb 28.
Salmonella is the main human pathogen present in the poultry chain. Salmonella Heidelberg is one of the most important serovars for public health since it has been frequently isolated in broiler chickens from different countries and may present multidrug resistance (MDR). This study was carried out with 130 S. Heidelberg isolates collected from pre-slaughter broiler farms in 2019 and 2020 in 18 cities from three Brazilian states to study relevant aspects regarding their genotypic and phenotypic resistance. The isolates were tested and identified using somatic and flagellar antiserum (0:4, H:2, and H:r), and an antimicrobial susceptibility test (AST) was performed against 11 antibiotics for veterinary use. The strains were typed by Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR, and representatives of the main clusters of the identified profiles were sequenced by Whole Genome Sequencing (WGS). AST results showed that all isolates were resistant to sulfonamide, 54 % (70/130) were resistant to amoxicillin, and only one was sensitive to tetracycline. Twelve isolates (15.4 %) were MDR. The dendrogram obtained from the ERIC-PCR showed that the strains were grouped into 27 clusters with similarity above 90 %, with some isolates showing 100 % similarity but with different phenotypic profiles of antimicrobial resistance. Identical strains collected on the same farm on other dates were identified, indicating that they were residents. WGS identified 66 antibiotic-resistance genes. The sul2 (present in all sequenced samples) and tet(A) genes were highlighted and validated in the experimental analysis. The fosA7 gene was also identified in all sequenced samples, but resistance was not observed in the phenotypic test, possibly due to the heteroresistance of the S. Heidelberg strains evaluated. Considering that chicken meat is one of the most consumed meats in the world, the data obtained in the present study can corroborate the mapping of the origin and trends of antimicrobial resistance.
沙门氏菌是家禽产业链中主要的人类病原体。海德堡沙门氏菌是对公共卫生最重要的血清型之一，因为它经常在来自不同国家的肉鸡中分离出来，并且可能呈现多重耐药性（MDR）。本研究对2019年和2020年从巴西三个州的18个城市的屠宰前肉鸡养殖场收集的130株海德堡沙门氏菌分离株进行了研究，以探讨其基因型和表型耐药性的相关方面。使用菌体和鞭毛抗血清（0:4、H:2和H:r）对分离株进行检测和鉴定，并对11种兽用抗生素进行了药敏试验（AST）。通过肠杆菌重复基因间共识（ERIC）-PCR对菌株进行分型，并通过全基因组测序（WGS）对鉴定出的主要谱系簇的代表菌株进行测序。AST结果显示，所有分离株均对磺胺类药物耐药，54%（70/130）对阿莫西林耐药，只有一株对四环素敏感。12株分离株（15.4%）为多重耐药。从ERIC-PCR获得的树状图显示，菌株被分为27个相似性高于90%的簇，一些分离株显示100%的相似性，但具有不同的抗菌药物耐药表型。在同一养殖场其他日期收集到相同菌株，表明它们是常驻菌。WGS鉴定出66个抗生素耐药基因。sul2（存在于所有测序样本中）和tet(A)基因在实验分析中得到突出和验证。在所有测序样本中也鉴定出了fosA7基因，但在表型试验中未观察到耐药性，这可能是由于所评估的海德堡沙门氏菌菌株存在异质性耐药。鉴于鸡肉是世界上消费最多的肉类之一，本研究获得的数据可以证实抗菌药物耐药性的来源和趋势图谱。
