Sato H, Hattori S, Ishida N, Imamura Y, Kawakita M
Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo, Japan.
Virus Res. 1987 Sep;8(3):217-32. doi: 10.1016/0168-1702(87)90017-7.
The nucleotide sequence of DNA clones complementary to the genomic RNA of an extremely avirulent strain D26 of Newcastle disease virus was analyzed, and the sequence of 2102 nucleotides directly following F gene reported previously (Sato et al., 1987, Virus Res. 7, 241-255), and corresponding to HN0 gene was determined. A long open reading frame coding for the HN0 peptide of 616 amino acid residues was found in this sequence. It was flanked by the consensus sequences N1 and N2 (Ishida et al., 1986, Nucleic Acids Res. 14, 6551-6564), and the former was shown by the primer extension method to serve as the transcriptional initiation site. The deduced amino acid sequence of the HN0 peptide was highly homologous to that of the HN peptides of strains Beaudette C and B1, but had a carboxyl terminal extension of 39 amino acid residues with a potential glycosylation site in it. The terminal extension is likely to be excised during the processing, and this is consistent with the observation that unglycosylated HN0 is larger in size than unglycosylated HN. A microheterogeneity among the cDNA clones in the nucleotide sequence was also noted which may be relevant to the synthesis of a small amount of an HN-sized peptide in strain D26-infected cells.
分析了与新城疫病毒极弱毒株D26基因组RNA互补的DNA克隆的核苷酸序列，确定了先前报道的（佐藤等人，1987年，病毒研究7，241 - 255）紧跟F基因之后的2102个核苷酸的序列，该序列对应于HN0基因。在该序列中发现了一个编码616个氨基酸残基的HN0肽的长开放阅读框。它两侧是共有序列N1和N2（石田等人，1986年，核酸研究14，6551 - 6564），并且通过引物延伸法表明前者作为转录起始位点。推导的HN0肽的氨基酸序列与Beaudette C和B1株的HN肽高度同源，但有一个39个氨基酸残基的羧基末端延伸，其中有一个潜在的糖基化位点。末端延伸可能在加工过程中被切除，这与未糖基化的HN0比未糖基化的HN尺寸更大的观察结果一致。还注意到cDNA克隆在核苷酸序列上存在微异质性，这可能与D26感染细胞中少量HN大小肽的合成有关。
