阿格里省附近地区蜗牛物种中幼虫阶段的分子流行情况
Molecular Prevalence of Larval Stages of in Species Snails in the Vicinity of the Ağrı Province.
作者信息
Ünlü Ahmet Hakan, Yıldız Rahmi, Aydemir Selahattin, Ekici Abdurrahman
机构信息
Van Yüzüncü Yıl Üniversitesi, Gevaş Meslek Yüksekokulu, Veterinerlik Bölümü, Van, Türkiye
Van Yüzüncü Yıl Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Van, Türkiye
出版信息
Turkiye Parazitol Derg. 2023 Mar 2;47(1):34-37. doi: 10.4274/tpd.galenos.2022.52714.
OBJECTIVE
known as the great pond snail, is one of the intermediate hosts of , a zoonotic parasite. In this study, it was aimed to determine the larval forms of by polymerase chain reaction (PCR) in species snails collected from the vicinity of Ağrı province.
METHODS
In this study, 150 snails were collected from the Ağrı province. The freshwater snails brought to the laboratory were dissected, then their soft tissues were examined under a microscope. DNA extraction was performed on the dissected snails. After DNA extraction, PCR was performed using primers targeting the cytochrome c oxidase subunit 1 gene region.
RESULTS
In the microscopic examination, larval forms of could not be detected. However, it was concluded that two (1.3%) freshwater snails were infected with the larval forms of in the PCR.
CONCLUSION
It was determined that served as an intermediate host to in the study area.
目的
大瓶螺，又称福寿螺，是一种人畜共患寄生虫的中间宿主之一。本研究旨在通过聚合酶链反应（PCR）确定从阿格里省附近采集的150种蜗牛中该寄生虫的幼虫形态。
方法
本研究从阿格里省采集了150只福寿螺。将带到实验室的淡水螺解剖，然后在显微镜下检查其软组织。对解剖后的蜗牛进行DNA提取。DNA提取后，使用针对细胞色素c氧化酶亚基1基因区域的引物进行PCR。
结果
在显微镜检查中未检测到该寄生虫的幼虫形态。然而，通过PCR得出结论，有两只（1.3%）福寿螺感染了该寄生虫的幼虫形态。
结论
在研究区域确定福寿螺是该寄生虫的中间宿主。
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